The rod shaped cells of Bacillus megatarium are converted to spherical units called protoplasts on treatment with the enzyme
(3) cellulase and hemicellulase
(4) cellulase, hemicellulase and pectinase
(1) Protoplast is a sphere remaining after gram-positive bacteria have their cell contents lysed by lysozymes. The bacterial cell wall constituents are absent. In gram-negative organisms these spheres retain an outer wall layer.