NEET Zoology Biotechnology Principles and Processes Questions Solved

NEET - 2010

Restriction endonucleases are enzymes which
(a) make cuts at specific positions within the DNA molecule
(b) recognize a specific nucleotide sequence for binding of DNA ligase
(c) restrict the action of the enzyme DNA polymerase
(d) remove nucleotides from the ends of the DNA molecule

(a) Restriction endonuclease recognizes a specific DNA base sequence (recognition sequence or recognition site, restriction sequence or restriction site) and cleaves both the strands of DNA at or near that site. The enzyme cuts the DNA, generating restriction fragments with overhanging ends or blunt ends.

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