Continuous addition of sugars in ‘fed batch’ fermentation is done to

(a) obtain antibiotics

(b) purify enzymes

(c) degrade sewage

(d) produce methane

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Which one of the following is used as vector for cloning genes into higher organisms?

(a) Baculovirus

(b) Salmonella typhimurium

(c) Rhizopus nigricans

(d) Retrovirus

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Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

(a) 5'-CGTTCG-3'

     3'-ATGGTA--5'

(b) 5'-GATATG-3'

     3'-CTACTA-5'

(c) 5'-GAATTC-3'

     3'-CTTAAG-5'

(d) 5'-CACGTA-3'

     3'-CTCAGT-5'

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DNA or RNA segment tagged with a radioactive molecule is called

(a) vector

(b) probe

(c) clone

(d) plasmid

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Restriction endonucleases are enzymes which
(a) make cuts at specific positions within the DNA molecule
(b) recognize a specific nucleotide sequence for binding of DNA ligase
(c) restrict the action of the enzyme DNA polymerase
(d) remove nucleotides from the ends of the DNA molecule

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Which one of the following is commonly used in transfer of foreign DNA into crop plants?

(a) Trichoderma harzianum

(b) Meloidogyne incognita

(c) Agrobacterium tumefaciens

(d) Penicillium expansum

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Polyethylene glycol method is used for

(a) gene transfer without a vector

(b) biodiesel production

(c) seedless fruit production

(d) energy production from sewage

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Gel electrophoresis is used for

(a) cutting of DNA into fragments

(b) separation of DNA fragments according to their size

(c) construction of recombinant DNA by joining with cloning vectors

(d) isolation of DNA molecule

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Human insulin is being commercially produced from a transgenic species of

(a) Escherichia

(b) Mycobacterium

(c) Rhizobium

(d) Saccharomyces

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The linking of antibiotic resistance gene with the plasmid vector became possible with

(a) DNA ligase               (b) endonucleases

(c) DNA polymerase       (d) exonucleases

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