The DNA molecule to which the gene of interest is integrated for cloning is called:

1.	Transformer	2.	Vector
3.	Template	4.	Carrier

Which vector can clone only a small fragment of DNA?

1. Bacterial artificial chromosome

2. Yeast artificial chromosome

3. Plasmid

4. Cosmid

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

(1) centrifugation

(2) polymerase chain reaction

(3) electrophoresis

(4) restriction mapping

Which of the following is not correctly matched for the organism and its cell wall

degrading enzyme?

1. Bacteria —Lysozyme

2. Plant cells — Cellulase

3. Algae —Methylase

4. Fungi — Chitinase

The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)?

1. Ori-original restriction enzyme

2. rop-reduced osmotic pressure

3. Hind III, Eco RI -selectable markers

4. amp^R, tet^R-antibiotic resistance genes

PCR and Restriction Fragment Length Polymorphism are the methods for

1. study of enzymes

2. genetic transformation

3. DNA sequencing

4. genetic fingerprinting

A single strand of nucleic acid tagged with a radioactive molecule is called

1. vector

2. selectable marker

3. plasmid

4. probe

Which one is a true statement regarding DNA polymerase used in PCR?

1. It is used to ligate introduced DNA in recipient cells

2. It serves as a selectable marker

3. It is isolated from a virus

4. It remains active at high temperature

For transformation, microparticles coated with DNA to be bombarded with gene gun are

made up of

1. silver or platinum

2. platinum or zinc

3. silicon or platinum

4. gold or tungsten

Given below is a sample of portion of DNA strand giving the base sequence on the

opposite strands. What is so, special shown in it?

5'__ GAATTC_ 3'

3' __ CTTAAG__5'

1. Deletion mutation

2. Start codon at the 5' end

3. Palindromic sequence of base pairs

4. Replication completed