The Taq polymerase enzyme is obtained from

(a) Thiobacillus ferroxidans

(b) Bacillus subtilis

(c) Pseudomonas subtilis

(d) Thermus aquaticus

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The cutting of DNA at specific locations became possible with the discovery of 

(1) restriction enzymes

(2) probes

(3) selectable markets

(4) ligases

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The DNA molecule to which the gene of interest is integrated for cloning is called

(a) Transformer

(b) Vector

(c) Template

(d) Carrier 

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Which vector can clone only a small fragment of DNA?

(a) Bacterial artificial chromosome

(b) Yeast artificial chromosome

(c) Plasmid

(d) Cosmid

Concept Questions :-

Tools: Vectors: I
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DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

(1) centrifugation

(2) polymerase chain reaction

(3) electrophoresis

(4) restriction mapping

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Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?

(a) Bacteria —Lysozyme

(b) Plant cells — Cellulase

(c) Algae —Methylase

(d) Fungi — Chitinase

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The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of 

(1) Non-recombinant bacteria containing β-galactosidase

(2) Insertional inactivation of α-galactosidase in non-recombinant bacteria

(3) Insertional inactivation of α-galactosidase in recombinant bacteria

(4) Inactivation of glycosidase enzyme in recombinant bacteria

 

Concept Questions :-

Selectable Markers: II
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The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)?

(a) Ori-original restriction enzyme

(b) rop-reduced osmotic pressure

(c) Hind III, Eco RI -selectable markers

(d) ampR, tetR-antibiotic resistance genes

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PCR and Restriction Fragment Length Polymorphism are the methods for

(a) study of enzymes

(b) genetic transformation

(c) DNA sequencing

(d) genetic fingerprinting

Concept Questions :-

Large Scale Production: I
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A single strand of nucleic acid tagged with a radioactive molecule is called

(a) vector

(b) selectable marker

(c) plasmid

(d) probe

 

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