Which of the following restriction enzymes produces blunt ends?

(1) Sal I                           

(2) Eco RV

(3) Xho                           

(4) Hind III

 

Which of the following is a endonuclease?

(1) Protease

(2) DNase I

(3) RNase restriction

(4) Hind II

The Taq polymerase enzyme is obtained from

1. Thiobacillus ferroxidans

2. Bacillus subtilis

3. Pseudomonas subtilis

4. Thermus aquaticus

The cutting of DNA at specific locations became possible with the discovery of 

(1) restriction enzymes

(2) probes

(3) selectable markets

(4) ligases

The DNA molecule to which the gene of interest is integrated for cloning is called:

Which vector can clone only a small fragment of DNA?

1. Bacterial artificial chromosome

2. Yeast artificial chromosome

3. Plasmid

4. Cosmid

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

(1) centrifugation

(2) polymerase chain reaction

(3) electrophoresis

(4) restriction mapping

Which of the following is not correctly matched for the organism and its cell wall

degrading enzyme?

1. Bacteria —Lysozyme

2. Plant cells — Cellulase

3. Algae —Methylase

4. Fungi — Chitinase

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of 

(1) Non-recombinant bacteria containing $\beta$-galactosidase

(2) Insertional inactivation of $\alpha$-galactosidase in non-recombinant bacteria

(3) Insertional inactivation of β-galactosidase in recombinant bacteria

(4) Inactivation of glycosidase enzyme in recombinant bacteria

The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)?

1. Ori-original restriction enzyme

2. rop-reduced osmotic pressure

3. Hind III, Eco RI -selectable markers

4. amp^R, tet^R-antibiotic resistance genes