Q. No.Which of the following is not a component of downstream processing?
1. Separation
2. Purification
3. Preservation
4. Expression
The DNA molecule to which the gene of interest is integrated for cloning is called:
| 1. | Transformer | 2. | Vector |
| 3. | Template | 4. | Carrier |
Stirred-tank bioreactors have been designed for
| 1. | addition of preservatives of the product |
| 2. | purification of the product |
| 3. | ensuring anaerobic conditions in the culture vessel |
| 4. | availability of oxygen throughout the process |
The DNA fragments separated on an agarose gel can be visualized after staining with:
| 1. | Acetocarmine | 2. | Aniline blue |
| 3. | Ethidium bromide | 4. | Bromophenol blue |
1. Extension, Denaturation, Annealing
2. Annealing, Extension, Denaturation
3. Denaturation, Extension, Annealing
4. Denaturation, Annealing, Extension
The process of separation and purification of expressed protein before marketing is called:
1. Downstream processing
2. Bioprocessing
3. Post-product processing
4. Upstream processing
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using?
| 1. | Eco Rl | 2. | taq polymerase |
| 3. | polymerase lll | 4. | ligase |
DNA fragments are:
1. Negatively charged
2. Neutral
3. Either positively charged depending on their size
4. Positively charged
A gene whose expression helps to identify transformed cell is known as:
1. Vector
2. Plasmid
3. Structural gene
4. Selectable marker
What is the criterion for the movement of DNA fragments on agarose gel during gel electrophoresis?
| 1. | The smaller the fragment size, the farther it moves |
| 2. | Positively charged fragments move to farther end |
| 3. | Negatively charged fragments do not move |
| 4. | The larger the fragment size, the farther it moves |
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