Q. No.The Taq polymerase enzyme is obtained from
1. Thiobacillus ferroxidans
2. Bacillus subtilis
3. Pseudomonas subtilis
4. Thermus aquaticus
The cutting of DNA at specific locations became possible with the discovery of
(1) restriction enzymes
(2) probes
(3) selectable markets
(4) ligases
Which of the following is not correctly matched for the organism and its cell wall
degrading enzyme?
1. Bacteria —Lysozyme
2. Plant cells — Cellulase
3. Algae —Methylase
4. Fungi — Chitinase
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
(1) Eco RI
(2) taq polymerase
(3) polymerase III
(4) ligase
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by
| 1. | centrifugation |
| 2. | polymerase chain reaction |
| 3. | electrophoresis |
| 4. | restriction mapping |
The DNA molecule to which the gene of interest is integrated for cloning is called:
| 1. | Transformer | 2. | Vector |
| 3. | Template | 4. | Carrier |
Which of the following is a endonuclease?
(1) Protease
(2) DNase I
(3) RNase restriction
(4) Hind II
Which of the following is not a component of downstream processing?
1. Separation
2. Purification
3. Preservation
4. Expression
Which vector can clone only a small fragment of DNA?
1. Bacterial artificial chromosome
2. Yeast artificial chromosome
3. Plasmid
4. Cosmid
Which of the following restriction enzymes produces blunt ends?
1. Sal I
2. Eco RV
3. Xho
4. Hind III
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