Stirred-tank bioreactors have been designed for:

(a) purification of product

(b) addition of preservatives to the product

(c) availability of oxygen throughout the process

(d) ensuring anaerobic conditions in the culture vessel

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A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

(a) Eco RI                       (b) taq polymerase

(c) polymerase III           (d) ligase

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Which of the following is not a component of downstream processing?

(a) Separation

(b) Purification

(c) Preservation

(d) Expression

Concept Questions :-

Large Scale Production: II
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Which of the following restriction enzymes produces blunt ends?

(a) Sal I                           (b) Eco RV

(c) Xho                            (d) Hind III

 

Concept Questions :-

Tools: Enzymes:II
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Which of the following is a endonuclease?

(a) Protease

(b) DNase I

(c) RNase restriction

(d) Hind II

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The Taq polymerase enzyme is obtained from

(a) Thiobacillus ferroxidans

(b) Bacillus subtilis

(c) Pseudomonas subtilis

(d) Thermus aquaticus

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The cutting of DNA at specific locations became possible with the discovery of 

(1) restriction enzymes

(2) probes

(3) selectable markets

(4) ligases

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The DNA molecule to which the gene of interest is integrated for cloning is called

(a) Transformer

(b) Vector

(c) Template

(d) Carrier 

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Which vector can clone only a small fragment of DNA?

(a) Bacterial artificial chromosome

(b) Yeast artificial chromosome

(c) Plasmid

(d) Cosmid

Concept Questions :-

Tools: Vectors: I
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DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

(1) centrifugation

(2) polymerase chain reaction

(3) electrophoresis

(4) restriction mapping

Please attempt this question first.

Difficulty Level: