What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?
(a) The larger the fragment size, the farther it moves
(b) The smaller the fragment size, the farther it moves
(c) Positively charged fragments move to farther end
(d) Negatively charged fragments do not move
(b) Gel electrophoresis is used for the separation of molecules of similar electric charge on the basis of their size. Hence, smaller the DNA fragment size the farther it moves. Thinking Process Agarose gel matrix functions as sieve. Smaller DNA fragments easily move and larger fragments take time to move in gel matrix.