The living organisms can be unexceptionally distinguished from the non-living things on the basis of their ability for:
(1) responsiveness to touch
(2) interaction with the environment and progressive evolution
(4) growth and movement
Considering, unexceptionally means without exception, option (2) is correct, as reproduction is not shown by worker honey bees which are living organisms. Not all living organisms response to touch. All living organisms do interact with environment and show evolution. Non living things might interact with environment but will not show evolution.