The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of which of the following organism?
1. | Escherichia coli | 2. | Bacillus thuringiensis |
3. | Salmonella typhimurium | 4. | Agrobacterium tumefaciens |
In a cell, the separation of DNA strands is brought about by the enzyme DNA helicase, whereas in PCR, the separation of DNA strands is due to :
1. High temperature
2. Two sets of Primers
3. Taq DNA polymerase
4. Deoxynucleotides
Identify the properties of a good vector used in rDNA technology:
(a) | It should have origin of replication supporting a high copy number |
(b) | It should have preferably more than '2' recognition sites |
(c) | The restriction sites in vector should be in the antibiotic- resistant genes |
(d) | It should have suitable marker genes |
(e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
1. | (a), (c) and (e) only | 2. | (c), (d) and (e) only |
3. | (a), (b) and (c) only | 4. | (a), (c), (d) and (e) only |