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Q. No.Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A):
In rDNA technology non-recombinants transformed bacteria grow on the medium containing ampicillin as well as medium containing tetracycline
Reason (R):
Recombinant plasmids contain the foreign gene of interest.
In the light of the above statements, choose the correct answer from the options given below:
1.
(A) is correct but (R) is not correct.
2.
(A) is not correct but (R) is correct.
3.
Both (A) and (R) are correct and (R) is the correct explanation of (A).
4.
Both (A) and (R) are correct but (R) is not the correct explanation of (A).
In the light of the above statements, choose the correct answer from the options given below:
Subtopic: General Design of an rDNA experiment |
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The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of which of the following organism?
| 1. | Escherichia coli | 2. | Bacillus thuringiensis |
| 3. | Salmonella typhimurium | 4. | Agrobacterium tumefaciens |
Subtopic: Process of Biotech | Introduction & History |
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NEET - 2022
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In a cell, the separation of DNA strands is brought about by the enzyme DNA helicase, whereas in PCR, the separation of DNA strands is due to :
1. High temperature
2. Two sets of Primers
3. Taq DNA polymerase
4. Deoxynucleotides
Subtopic: Polymerase Chain Reaction: PCR |
66%
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NEET - 2022
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Identify the properties of a good vector used in rDNA technology:
| (a) | It should have origin of replication supporting a high copy number |
| (b) | It should have preferably more than '2' recognition sites |
| (c) | The restriction sites in vector should be in the antibiotic- resistant genes |
| (d) | It should have suitable marker genes |
| (e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
| 1. | (a), (c) and (e) only | 2. | (c), (d) and (e) only |
| 3. | (a), (b) and (c) only | 4. | (a), (c), (d) and (e) only |
Subtopic: Cloning Vector | Tools |
86%
From NCERT
NEET - 2022
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A:
Insertional inactivation method helps to differentiate recombinant from non-recombinant
R: Selection of recombinant in this method depends upon inactivation of antibiotics
In the following questions a statement of assertion (A) is followed by a statement of reason (R).
1. If both Assertion & Reason are true and the reason is the correct explanation of the assertion, then mark (1)
2. If both Assertion & Reason are true but the reason is not the correct explanation of the assertion, then mark (2)
3. If Assertion is true statement but Reason is false, then mark (3)
4. If both Assertion and Reason are false statements, then mark (4)
R: Selection of recombinant in this method depends upon inactivation of antibiotics
In the following questions a statement of assertion (A) is followed by a statement of reason (R).
1. If both Assertion & Reason are true and the reason is the correct explanation of the assertion, then mark (1)
2. If both Assertion & Reason are true but the reason is not the correct explanation of the assertion, then mark (2)
3. If Assertion is true statement but Reason is false, then mark (3)
4. If both Assertion and Reason are false statements, then mark (4)
- If both the assertion and the reason are true and the reason is a correct explanation of the assertion
- If both the assertion and reason are true but the reason is not a correct explanation of the assertion
- If the assertion is true but the reason is false
- If both the assertion and reason are false
Subtopic: Selection of Recombinant Transformants |
From NCERT
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Which of the following is true about Stanley cohen and Herbet Boyer experiment ?
1. They used plasmid of salmonella typhimurium as a vector
2. They used plasmid of Escherichia coli to obtained antibiotic resistance gene
3. They used molecular scissor’s to obtained antibiotic resistance gene from the plasmid of salmonella typhimurium
4. They used DNA ligase to join antibiotic resistance gene obtained from Escherichia coli to the plasmid of salmonella
1. They used plasmid of salmonella typhimurium as a vector
2. They used plasmid of Escherichia coli to obtained antibiotic resistance gene
3. They used molecular scissor’s to obtained antibiotic resistance gene from the plasmid of salmonella typhimurium
4. They used DNA ligase to join antibiotic resistance gene obtained from Escherichia coli to the plasmid of salmonella
Subtopic: Restriction Enzymes - Main Enzymes |
From NCERT
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Gene transfer involves some essential stages such as
(i) inserting recombinant DNA into suitable recipient organism
(ii) isolation of a fragment of DNA
(iii) screening of transformed cells
(iv) integrating DNA fragment into suitable vector
The experimental sequence of these events should be
1. (i), (ii), (iii), (iv)
2. (ii), (iv), (i), (iii)
3. (iv), (i), (ii), (iii)
4. (i), (iii), (ii), (iv).
(i) inserting recombinant DNA into suitable recipient organism
(ii) isolation of a fragment of DNA
(iii) screening of transformed cells
(iv) integrating DNA fragment into suitable vector
The experimental sequence of these events should be
1. (i), (ii), (iii), (iv)
2. (ii), (iv), (i), (iii)
3. (iv), (i), (ii), (iii)
4. (i), (iii), (ii), (iv).
Subtopic: Transforming Plant & Animal Cell |
83%
From NCERT
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Consider the following steps which are involved in cloning of foreign DNA into plasmid vector
A. Isolation of foreign DNA
B. Ligation of DNA to plasmid
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A > B > C > E > D
2. A > B > E > C > D
3. A > C > B > E > D
4. A > D > E > B > C
A. Isolation of foreign DNA
B. Ligation of DNA to plasmid
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A > B > C > E > D
2. A > B > E > C > D
3. A > C > B > E > D
4. A > D > E > B > C
Subtopic: Tools | General Design of an rDNA experiment |
74%
From NCERT
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Which of the following DNA is used by Agrobacterium tumefaciens to transform normal cells into a tumor?
1. B-DNA
2. Z-DNA
3. T-DNA
4. A-DNA
1. B-DNA
2. Z-DNA
3. T-DNA
4. A-DNA
Subtopic: Transforming Plant & Animal Cell |
88%
From NCERT
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Selection of recombinations based on their inability to produce colour in the presence of a chromogenic substrate is
1. Insertional inactivation
2. Insertional mutagenesis
3. Insertional selection
4. Insertional chromogenesis
1. Insertional inactivation
2. Insertional mutagenesis
3. Insertional selection
4. Insertional chromogenesis
Subtopic: Cloning Vector |
89%
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