ds RNA can be used during which one of the following ?
1. Gene sequencing
2. Polymerase Chain Reaction
3. Gene silencing
4. Autoradiography
| 1. | Genetically engineered lymphocytes are not immortal cells. |
| 2. | Retroviral vector is introduced into these lymphocytes. |
| 3. | Gene isolated from marrow cells producing ADA is introduced into cells at embryonic stages |
| 4. | Lymphocytes from patient's blood are grown in culture, outside the body. |
| (a) | Pro-hormone insulin contain extra stretch of C-peptide |
| (b) | A-peptide and B-peptide chains of insulin were produced separately in E.coli, extracted and combined by creating disulphide bond between them. |
| (c) | Insulin used for treating Diabetes was extracted from Cattles and Pigs. |
| (d) | Pro-hormone Insulin needs to be processed for converting into a mature and functional hormone. |
| (e) | Some patients develop allergic reactions to the foreign insulin. |
Genetically engineered insulin for human is produced from:
1. Escherichia coli
2. Psuedomonas putida
3. Bacillus thuringiensis
4. Rhizobium meliloti
Give below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
| Assertion (A): | In human beings, insulin is synthesized as a pro-hormone which needs to be processed before it becomes fully mature and functional. |
| Reason (R): | The extra stretch of C-peptide is to be removed from A and B peptide chain of insulin. |
In the light of the above statements, Choose the most appropriate answer from the option given below :
| 1. | (A) is True but (R) is False. |
| 2. | (A) is False but (R) is True. |
| 3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
Given below are two statements: one is labeled as Assertion (A) and the other is labeled as Reason (R).
| Assertion (A): | The nematode cannot survive in a transgenic host which expresses specific interfering RNA. |
| Reason (R): | Nematode-specific gene introduced in the host produces both sense and antisense complementary RNA which initiate RNA interference in the host cell. |
In light of the above statement, choose the most appropriate answer from the option given below :
| 1. | (A) is correct but (R) is not correct. |
| 2. | (A) is not correct but (R) is correct. |
| 3. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
| 1. | Gene therapy – Use of retrovirus to introduce cDNA into the host cell. |
| 2. | PCR – Used to detect mutations is genes in suspected cancer patients |
| 3. | ELISA – Detection of non-proteinecious antigens. |
| 4. | Probe – A single strand DNA or RNA, tagged with radioactive molecule, will never hybridized with mutated gene |
| Assertion(A): | Agrobacterium tumefaciens is popular in genetic engineering because this bacterium is associated with roots of pulse crops. |
| Reason(R): | A gene incorporated in the bacterial chromosomal genome gets automatically transferred to the crop with which the bacterium is associated |
In the following questions a statement of assertion (A) is followed by a statement of reason (R).
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are False. |