Which of the following restriction sites is located within the gene for tetracycline resistance in the plasmid pBR322?

1. BamHI

2. Psti

3. Clal

4. Pvull

The technique not used for transformation of plant cells in recombinant procedures is:

1. Biolistics

2. Agrobacterium mediation

3. Use of viruses

4. Micro-injection

In the screening process during rDNA experiments, clones that metabolize $\beta$-gal turn:

1. Colorless

2. Blue

3. Yellow

4. Green

Microinjection is suitable for :

1. Injecting an ovum into the sperm in IVF

2. Transforming animal cells

3. Injecting very small sized drug particles into neurons

4. Conferring antibiotic resistance to a certain strain of bacteria 

The plasmid pBR322 does not contain:

Restriction enzymes are synthesized by:

1. Bacteria only

2. Yeast and bacteria only

3. Eukaryotic cells only

4. All kinds of cells

The bioreactor shown in the diagram is:

1. Sparged stirred tank type

2. Airlift type

3. Simple stirred tank type

4. Fluidized bed type

What is true about the steps of PCR shown in the diagram given below?

1. l and ll only
2. l and lll only
3. ll and lll only
4. l, ll and lll

A and B in the pBR 322, shown in the diagram given below, respectively represent recognition sequences of:

1. BamH I and Sma I

2. Hind II and Sma I

3. BamH I and SaI I

4. SaI I and Hind II

What is true A,B and C in the given diagrammatic representation of rDNA technology ?