When isolating the pure DNA from a bacterial cell, the cell should not be treated with:

1. lysozyme

2. proteases

3. ribonuclease

4. deoxyribonuclease

Concept Questions :-

Host & Desired DNA: I
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A host cell normally does not take up a foreign DNA until it has been made competent to do so. This is because:

1. DNA is a hydrophilic molecule

2. DNA is a very large molecule

3. there are no receptors for DNA on the cell membrane

4. DNA is an inert molecule

Concept Questions :-

Host & Desired DNA: II
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Taq polymerase is used in the polymerase chain reaction because:

(1) It replicates DNA faster than other enzymes

(2) It is the only enzyme that can replicate DNA invitro

(3) It does not require an RNA primer to function

(4) It is thermostable 

Concept Questions :-

Large Scale Production: I
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The first type II restriction endonuclease discovered that could cut dsDNA specific site was:

1. EcoRI

2. SmaI

3. Hind II

4. Hind III

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Which statement regarding restriction endonucleases is NOT correct?

1. They recognize a specific base sequence in the DNA.

2. They are produced by bacterial cells as a primitive immune system.

3. They digest DNA by removing nucleotides from a free 3' end.

4. They often generate stick ends.

Concept Questions :-

Tools: Enzymes:II
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The first type II restriction endonuclease discovered that could cut dsDNA at specific site was:

1. EcoRI                        2. SmaI

3. Hind II                      4. Hind III

Concept Questions :-

Tools: Enzymes:II
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What limitation of traditional hybridization used for plant and animal breeding has been overcome by

the use of rDNA techniques?

1. High cost

2. Insertion of undesirable genes

3. Complexity of the procedure

4. Antigenicity of the product

Concept Questions :-

Intro & History: II
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Which of the following restriction sites is located within the gene for tetracycline resistance in the plasmid pBR322?

1. BamHI

2. Psti

3. Clal

4. Pvull

Concept Questions :-

Tools: Vectors:II
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The technique not used for transformation of plant cells in recombinant procedures is:

1. Biolistics

2. Agrobacterium mediation

3. Use of viruses

4. Micro-injection

Concept Questions :-

Tools: Vectors:II
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In the screening process during rDNA experiments, clones that metabolize β-gal turn:

1. Colorless

2. Blue

3. Yellow

4. Green

Concept Questions :-

Host & Desired DNA: I
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