Match the following columns and select the correct option:
| Column-I | Column-II | ||
| (i) | Typhoid | (a) | Haemophilus influenzae |
| (ii) | Malaria | (b) | Wuchereria bancrofti |
| (iii) | Pneumonia | (c) | Plasmodium vivax |
| (iv) | Filariasis | (d) | Salmonella typhi |
| Options: | (i) | (ii) | (iii) | (iv) |
| 1. | (d) | (c) | (a) | (b) |
| 2. | (c) | (d) | (b) | (a) |
| 3. | (a) | (c) | (b) | (d) |
| 4. | (a) | (b) | (d) | (c) |
The yellowish fluid "colostrum" secreted by mammary glands of the mother during the initial days of lactation has abundant antibodies (IgA) to protect the infant. This type of immunity is called as:
| 1. | Passive immunity | 2. | Active immunity |
| 3. | Acquired immunity | 4. | Autoimmunity |
First discovered restriction endonuclease that always cuts DNA molecules at a particular point by recognizing a specific sequence of six base pairs is:
1. EcoRI
2. Adenosine deaminase
3. Thermostable DNA polymerase
4. Hind II
In recombinant DNA technology antibiotics are used:
1. to keep medium bacteria-free
2. to detect alien DNA
3. to impart disease-resistance to the host plant
4. as selectable markers
Match the following techniques or instruments with their usage:
| (a) | Bioreactor | (i) | Separation of DNA fragments |
| (b) | Electrophoresis | (ii) | Production of large quantities of products |
| (c) | PCR | (iii) | Detection of pathogen, based on antigen-antibody reaction |
| (d) | ELISA | (iv) | Amplification of nucleic acids |
Select the correct option from the following:
| 1. | (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i) |
| 2. | (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii) |
| 3. | (a)-(iv), (b)-(iii), (c)-(ii), (d)-(i) |
| 4. | (a)-(ii), (b)-(i), (c)-(iii), (d)-(iv) |
In a mixture, DNA fragments are separated by-
1. Bioprocess engineering
2. Restriction digestion
3. Electrophoresis
4. Polymerase chain reaction
Spooling is:
| 1. | Amplification of DNA |
| 2. | Cutting of separated DNA bands from the agarose gel |
| 3. | Transfer of separated DNA fragments to synthetic membranes |
| 4. | Collection of isolated DNA |
Select the correct statement from the following:
| 1. | Gel electrophoresis is used for the amplification of a DNA segment. |
| 2. | The polymerase enzyme joins the gene of interest and the vector DNA. |
| 3. | Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions. |
| 4. | PCR is used for the isolation and separation of genes of interest. |
RNA interference is used for which of the following purposes in the field of biotechnology?
| 1. | to develop a plant tolerant to abiotic stresses |
| 2. | to develop a pest-resistant plant against infestation by nematode |
| 3. | to enhance the mineral usage by the plant |
| 4. | to reduce post-harvest losses |
The laws and rules to prevent unauthorized exploitation of bio-resources are termed as:
| 1. | Biopatenting | 2. | Bioethics |
| 3. | Bioengineering | 4. | Biopiracy |
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