Transplantation of tissues/organs fails often due to non-acceptance by the patient's body. Which type of immune-response is responsible for such rejections?
| 1. | Cell-mediated immune response |
| 2. | Hormonal immune response |
| 3. | Physiological immune response |
| 4. | Autoimmune response |
Coca alkaloid or cocaine is obtained from:
| 1. | Papaver somniferum | 2. | Atropa belladonna |
| 3. | Erythroxylum coca | 4. | Datura |
Match List-I with List-II
| List-I | List-II | ||
| (a) | Filariasis | (i) | Haemophilus influenzae |
| (b) | Amoebiasis | (ii) | Trichophyton |
| (c) | Pneumonia | (iii) | Wuchereria bancrofti |
| (d) | Ringworm | (iv) | Entamoeba histolytica |
Choose the correct answer from the options given below:
| (a) | (b) | (c) | (d) | |
| 1. | (i) | (ii) | (iv) | (iii) |
| 2. | (ii) | (iii) | (i) | (iv) |
| 3. | (iv) | (i) | (iii) | (ii) |
| 4. | (iii) | (iv) | (i) | (ii) |
Match the following enzymes with their functions:
|
Column-I |
Column-II |
||
|
(a) |
Restriction endonuclease |
(i) |
joins the DNA fragments |
|
(b) |
Exonuclease |
(ii) |
extends primers on genomic DNA template |
|
(c) |
DNA ligase |
(iii) |
cuts DNA at a specific position |
|
(d) |
Tag polymerase |
(iv) |
removes nucleotides from the ends of DNA |
Select the correct option from the following:
| Options: | (a) | (b) | (c) | (d) |
| 1. | (iii) | (i) | (iv) | (ii) |
| 2. | (iii) | (iv) | (i) | (ii) |
| 3. | (iv) | (iii) | (i) | (ii) |
| 4. | (ii) | (iv) | (i) | (iii) |
Given below are four statements pertaining to the separation of DNA fragments using gel electrophoresis. Identify the incorrect statements:
| (a) | DNA is a negatively charged molecule and so it is loaded on gel towards the anode terminal. |
| (b) | DNA fragments travel along the surface of the gel, whose concentration does not affect the movement of DNA. |
| (c) | The smaller the DNA fragment, the greater the distance it travels through it. |
| (d) | Pure DNA can be visualized directly by exposing it to UV radiation. |
Choose the correct answer from the options given below:
| 1. | (a), (c) and (d) | 2. | (a), (b) and (c) |
| 3. | (b), (c) and (d) | 4. | (a), (b) and (d) |
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactoside production and the recombinant plasmid is inserted in an E.coli strain,
| 1. | it will lead to the lysis of host cells. |
| 2. | it will be able to produce a novel protein with dual abilities. |
| 3. | it will not be able to confer ampicillin resistance to the host cell. |
| 4. | the transformed cells will have the ability to resist ampicillin as well as produce β -galactoside. |
Match the organism with its use in biotechnology.
| (a) | Bacillus thuringiensis | (i) | Cloning vector |
| (b) | Thermus aquaticus | (ii) | Construction of first rDNA molecule |
| (c) | Agrobacterium tumefaciens | (iii) | DNA polymerase |
| (d) | Salmonella typhimurium | (iv) | Cry proteins |
Select the correct option from the following:
| (a) | (b) | (c) | (d) | |
| 1. | (iv) | (iii) | (i) | (ii) |
| 2. | (iii) | (ii) | (iv) | (i) |
| 3. | (iii) | (iv) | (i) | (ii) |
| 4. | (ii) | (iv) | (iii) | (i) |
When gene targeting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as:
| 1. | Molecular diagnosis | 2. | Safety testing |
| 3. | Biopiracy | 4. | Gene therapy |
Nowadays, it is possible to detect the mutated gene causing cancer by allowing the radioactive probe to hybridise its complementary DNA in clone of cells, followed by its detection using autoradiography because:
| 1. | mutated gene does not appear on a photographic film as the probe has no complementarity with it. |
| 2. | mutated gene does not appear on a photographic film as the probe has complementarity with it. |
| 3. | mutated gene partially appears on a photographic film. |
| 4. | mutated gene completely and clearly appears on a photographic film. |
1. ELISA Technique
2. Hybridization Technique
3. Western Blotting Technique
4. Southern Blotting Technique
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