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Q. No.Read the following statements and choose the correct option.
Statement (A):
DNA cannot pass through cell membranes as it is a hydrophobic molecule.
Statement (B):
Treatment with Ca2+ ions increases the efficiency with which DNA enters into the bacterium.
1. Only statement A is correct
2. Only statement A is incorrect
3. Both A and B statements are correct
4. Both A and B statements are incorrect
Subtopic: Transforming Plant & Animal Cell |
63%
Level 2: 60%+
Hints
Match column-I with column-II and choose the correct option:
1. a(iv), b(ii), c(iii), d(i)
2. a(iv), b(ii), c(i), d(iii)
3. a(ii), b(iv), c(i), d(iii)
4. a(ii), b(iv), c(iii), d(i)
| Column-I | Column -II | ||
| a. | Biosynthetic stage | (i) | Increased surface area for oxygen transfer |
| b. | Downstream processing | (ii) | Large-scale production in the fermenter |
| c. | Sparged stirred-tank bioreactor |
(iii) | With foam breaker and flat bladed impeller |
| d. | Simple stirred-tank bioreactor |
(iv) | Separation and purification |
1. a(iv), b(ii), c(iii), d(i)
2. a(iv), b(ii), c(i), d(iii)
3. a(ii), b(iv), c(i), d(iii)
4. a(ii), b(iv), c(iii), d(i)
Subtopic: Bioreactors/Downstream Processing | Large Scale Production |
73%
Level 2: 60%+
Hints
Read the following statements and choose the correct option
Statement A: CaCl2 treatment increases the efficiency of DNA uptake to produce transformed bacterial cells
Statement B: The divalent Ca+2 ions create transient pores in the bacteria
1. Only statement A is correct
2. Only statement B is correct
3. Both A and B statements are correct
4. Both A and B statements are incorrect
Statement A: CaCl2 treatment increases the efficiency of DNA uptake to produce transformed bacterial cells
Statement B: The divalent Ca+2 ions create transient pores in the bacteria
1. Only statement A is correct
2. Only statement B is correct
3. Both A and B statements are correct
4. Both A and B statements are incorrect
Subtopic: Transforming Plant & Animal Cell |
Level 4: Below 35%
Hints
Read the following statements and choose the correct option
| Statement A: | Wine making as well as antigen production in recombinant organisms are included in biotechnology |
| Statement B: | Biotechnology is a very wide area which deals with techniques of using a natural organism (or its parts) as well as genetic engineering |
| 1. | Only statement A is correct |
| 2. | Only statement B is correct |
| 3. | Both statements A and B are correct |
| 4. | Both statements A and B are incorrect |
Subtopic: Introduction & History: I |
78%
Level 2: 60%+
Hints
If restriction endonuclease cuts the DNA a little away from the centre of the palindromic site, then what would happen?
Choose the correct option
1. (a) and (b) are correct
2. (a) and (c) are correct
3. (b) and (c) are correct
4. (a), (b) and (c) are correct
| (a) | The DNA fragments obtained will have 'sticky ends' |
| (b) | Construction of rDNA molecule would not be possible |
| (c) | Resultant DNA fragments with overhanging streches can be ligated with the help of DNA ligase |
Choose the correct option
1. (a) and (b) are correct
2. (a) and (c) are correct
3. (b) and (c) are correct
4. (a), (b) and (c) are correct
Subtopic: Restriction Enzymes - Main Enzymes |
82%
Level 1: 80%+
Hints
How many of the following features are correct with respect to plasmids?
1. Zero
2. Two
3. Three
4. One
| (a) | Extrachromosomal DNA molecule |
| (b) | Replicate independent of chromosomal DNA |
| (c) | Widely used as cloning vectors for animals host cells only |
2. Two
3. Three
4. One
Subtopic: Tools: Vectors: I | Tools: Vectors: II |
75%
Level 2: 60%+
Hints
Read the following statements and choose the correct option:
1. Both statements (A) and (B) are correct
2. Both statements (A) and (B) are incorrect
3. Only statement (A) is correct
4. Only statement (A) is incorrect
| Statement A: | Bacteriophages have very high copy numbers of their genome within the bacterial cells |
| Statement B: | To recover many copies of the target DNA, it should not be cloned in a vector whose origin supports high copy number |
1. Both statements (A) and (B) are correct
2. Both statements (A) and (B) are incorrect
3. Only statement (A) is correct
4. Only statement (A) is incorrect
Subtopic: Cloning Vector |
73%
Level 2: 60%+
Hints
Match the dyes listed in column I with their usage listed in column II and choose the correct option
1. a(iv), b(iii), c(i), d(ii)
2. a(ii), b(iv), c(i), d(iii)
3. a(iv), b(i), c(ii), d(iii)
4. a(iii), b(ii), c(i), d(iv)
| Column I | Column II | ||
| a. | Aniline blue | (i) | Used to stain DNA in agarose gel electrophoresis |
| b. | Bromophenol blue | (ii) | stains chromosomes |
| c. | Ethidium bromide | (iii) | Tracking dye |
| d. | Acetocarmine | (iv) | Used to stain collagen |
1. a(iv), b(iii), c(i), d(ii)
2. a(ii), b(iv), c(i), d(iii)
3. a(iv), b(i), c(ii), d(iii)
4. a(iii), b(ii), c(i), d(iv)
Subtopic: Tools |
57%
Level 3: 35%-60%
Hints
Read the following statements and choose the correct option:
1. Only statement A is correct
2. Only statement B is correct
3. Both A and B statements are correct
4. Both A and B statements are incorrect
| Statement A: | Agarose gel electrophoresis is employed to separate the fragments of DNA obtained after restriction enzyme digestion. |
| Statement B: | DNA is a negatively charged molecule and moves toward the cathode. |
1. Only statement A is correct
2. Only statement B is correct
3. Both A and B statements are correct
4. Both A and B statements are incorrect
Subtopic: Separation and Isolation of DNA fragments |
80%
Level 1: 80%+
Hints
| Assertion (A): | In vitro fertilisation, leading to a 'test-tube' baby, is included under the umbrella of biotechnology |
| Reason (R): | The definition of biotechnology, given by EFB encompasses only modern molecular biotechnology view |
Choose the correct answer from the options given below:
| 1. | (A) is true but (R) is false |
| 2. | (A) is false but (R) is true |
| 3. | Both (A) and (R) are true and (R) is the correct explanation of (A) |
| 4. | Both (A) and (R) are true but (R) is the not the correct explanation of (A) |
Subtopic: Introduction & History |
78%
Level 2: 60%+
Hints
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