Q. No.The cutting of DNA at specific locations became possible with the discovery of?
1. Restriction enzymes
2. Probes
3. Selectable markets
4. ligases
Which vector can clone only a small fragment of DNA?
1. Bacterial artificial chromosome
2. Yeast artificial chromosome
3. Plasmid
4. Cosmid
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:
1. Polymerase chain reaction
2. Electrophoresis
3. Restriction mapping
4. Centrifugation
1. Ori-original restriction enzyme
2. Rop-reduced osmotic pressure
3. Hind III, Eco RI-selectable markers
4. AmpR, tetiR-antibiotic resistance genes
Which one of the following is a case of wrong matching?
| 1. | Somatic hybridization- Fusion of two diverse cells |
| 2. | Vector DNA- Site for tRNA synthesis |
| 3. | Micropropagation- In vitro production of plants in large numbers |
| 4. | Callus - Unorganized mass of cells produced in tissue culture |
Which one is a true statement regarding DNA polymerase used in PCR?
| 1. | It is used to ligate introduces DNA in recipient cells |
| 2. | It serves as a selectable marker |
| 3. | It is isolated from a virus |
| 4. | It remains active at high temperatures |
For transformation, microparticles coated with DNA to be bombarded with gene gun are made up of:
1. Silver or platinum
2. Platinum or zinc
3. Silicon or platinum
4. Gold or tungsten
5’___GAATTC___3’
3’___CTTAAG__5’
1. Deletion mutation
2. Start codon at the 5' end
3. Palindromic sequence of base pairs
4. Replication completed
1. Coelom
2. Coenzyme
3. Coli
4. Colon
1. tissue culture
2. PCR
3. gel electrophoresis
4. spectrophotometry
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