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Q. No.According to the general account of rDNA technology, after the alien DNA has been linked with the vector DNA, the immediate next step involves:
1. Isolation of desired gene from the donor organism using restriction endonucleases
2. Extraction of the desired product and its downstream processing for commercial use
3. Transfer of recombinant DNA into the host organism where it multiplies
4. Amplification of gene of interest using PCR to generate multiple copies
1. Isolation of desired gene from the donor organism using restriction endonucleases
2. Extraction of the desired product and its downstream processing for commercial use
3. Transfer of recombinant DNA into the host organism where it multiplies
4. Amplification of gene of interest using PCR to generate multiple copies
Subtopic: Process of Biotech |
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In the polymerase chain reaction (PCR), the thermostable DNA polymerase used is:
1. A heat-stable RNA polymerase that synthesises DNA from RNA primers
2. A chemically modified form of E. coli DNA polymerase that remains stable at 95°C
3. Taq polymerase, which works optimally at 72°C and therefore requires cooling between each cycle
4. Isolated from T. aquaticus, can withstand high temperatures during denaturation
1. A heat-stable RNA polymerase that synthesises DNA from RNA primers
2. A chemically modified form of E. coli DNA polymerase that remains stable at 95°C
3. Taq polymerase, which works optimally at 72°C and therefore requires cooling between each cycle
4. Isolated from T. aquaticus, can withstand high temperatures during denaturation
Subtopic: Polymerase Chain Reaction: PCR |
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In the process of insertional inactivation [due to foreign DNA insertion] using pBR322 as a vector:
1. DNA insertion into any antibiotic resistance gene makes bacteria sensitive to both antibiotics
2. DNA is inserted into the origin of replication; recombinants cannot replicate in the host
3. DNA inserted into tetracycline resist. gene; recombinants grow on ampicillin but not on tetracycline
4. Recombinant bacteria lose the ability to take up the plasmid due to insertional inactivation
1. DNA insertion into any antibiotic resistance gene makes bacteria sensitive to both antibiotics
2. DNA is inserted into the origin of replication; recombinants cannot replicate in the host
3. DNA inserted into tetracycline resist. gene; recombinants grow on ampicillin but not on tetracycline
4. Recombinant bacteria lose the ability to take up the plasmid due to insertional inactivation
Subtopic: Cloning Vector |
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The 'ori' in a cloning vector is responsible for:
1. Initiating translation of foreign gene into protein product
2. Controlling the copy number of the linked DNA and initiating replication
3. Providing recognition sites for restriction endonuclease binding
4. Conferring antibiotic resistance to the host bacterial cell
1. Initiating translation of foreign gene into protein product
2. Controlling the copy number of the linked DNA and initiating replication
3. Providing recognition sites for restriction endonuclease binding
4. Conferring antibiotic resistance to the host bacterial cell
Subtopic: Cloning Vector |
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