According to the general account of rDNA technology, after the alien DNA has been linked with the vector DNA, the immediate next step involves:
1. Isolation of desired gene from the donor organism using restriction endonucleases
2. Extraction of the desired product and its downstream processing for commercial use
3. Transfer of recombinant DNA into the host organism where it multiplies
4. Amplification of gene of interest using PCR to generate multiple copies