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Q. No.In the process of insertional inactivation [due to foreign DNA insertion] using pBR322 as a vector:
1. DNA insertion into any antibiotic resistance gene makes bacteria sensitive to both antibiotics
2. DNA is inserted into the origin of replication; recombinants cannot replicate in the host
3. DNA inserted into tetracycline resist. gene; recombinants grow on ampicillin but not on tetracycline
4. Recombinant bacteria lose the ability to take up the plasmid due to insertional inactivation
1. DNA insertion into any antibiotic resistance gene makes bacteria sensitive to both antibiotics
2. DNA is inserted into the origin of replication; recombinants cannot replicate in the host
3. DNA inserted into tetracycline resist. gene; recombinants grow on ampicillin but not on tetracycline
4. Recombinant bacteria lose the ability to take up the plasmid due to insertional inactivation
Subtopic: Cloning Vector |
84%
Level 1: 80%+
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The 'ori' in a cloning vector is responsible for:
1. Initiating translation of foreign gene into protein product
2. Controlling the copy number of the linked DNA and initiating replication
3. Providing recognition sites for restriction endonuclease binding
4. Conferring antibiotic resistance to the host bacterial cell
1. Initiating translation of foreign gene into protein product
2. Controlling the copy number of the linked DNA and initiating replication
3. Providing recognition sites for restriction endonuclease binding
4. Conferring antibiotic resistance to the host bacterial cell
Subtopic: Cloning Vector |
87%
Level 1: 80%+
Please attempt this question first.
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Q. No.
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