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Q. No.During DNA replication, why does DNA polymerase make the lagging strand discontinuous?
1. Proofreading leaves gaps after excising mismatched base pairs
2. DNA polymerase sometimes falls off and has to start over
3. DNA polymerase only works 5' to 3'
4.DNA polymerase only works 3' to 5'
1. Proofreading leaves gaps after excising mismatched base pairs
2. DNA polymerase sometimes falls off and has to start over
3. DNA polymerase only works 5' to 3'
4.DNA polymerase only works 3' to 5'
Subtopic: DNA Replication |
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Level 2: 60%+
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Which of the following correctly describes regulatory sequences?
| 1. | Proteins that bind to regulatory molecules and initiate transcription. |
| 2. | Regions of DNA that interact with regulatory proteins to control transcription. |
| 3. | Ribosomal RNA molecules that regulate translation. |
| 4. | Structural genes that code for enzymes for metabolic pathways. |
Subtopic: Gene Regulation: Lac Operon |
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How does the enzyme reverse transcriptase violate the central dogma of molecular biology in HIV?
| 1. | Enzyme reverse transcriptase transcribes DNA to RNA, then again to DNA with no protein synthesis |
| 2. | The enzyme reverse transcriptase translates the RNA of the HIV into protein and then back to DNA. |
| 3. | The enzyme reverse transcriptase transcribes the DNA straight into the protein molecules. |
| 4. | The enzyme reverse transcriptase reverse transcribes the RNA in the genome of HIV to DNA. |
Subtopic: The DNA | DNA Double Helix |
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Which feature of the genetic code provides strong evidence for the common ancestry of all living organisms?
1. The code is degenerate
2. The code contains 64 codons.
3. The genetic code is almost universal.
4. The code contains stop codons
1. The code is degenerate
2. The code contains 64 codons.
3. The genetic code is almost universal.
4. The code contains stop codons
Subtopic: Genetic Code |
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Which biological process ensures that genetic information is faithfully passed on to the next generation?
1. DNA replication
2. RNA splicing
3. Transcription
4. Translation
1. DNA replication
2. RNA splicing
3. Transcription
4. Translation
Subtopic: DNA Replication |
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The first step in DNA fingerprinting begins with:
| 1. | DNA digestion with enzymes |
| 2. | DNA extraction |
| 3. | Sample collection (blood, saliva, hair root etc.) |
| 4. | Probe hybridisation |
Subtopic: DNA Fingerprinting |
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Once a biological sample is collected, what must be done before analysis?
| 1. | Southern blotting |
| 2. | DNA extraction and purification |
| 3. | DNA denaturation |
| 4. | Gel electrophoresis |
Subtopic: DNA Fingerprinting |
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Why is DNA purified at this stage?
| 1. | To make DNA fluorescent |
| 2. | To amplify DNA sequences |
| 3. | To prevent hybridisation with probes |
| 4. | To remove proteins and contaminants that interfere with enzyme action |
Subtopic: DNA Fingerprinting |
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Which tool is used to cut DNA into fragments at specific recognition sites ?
1. DNA ligase
2. Restriction endonuclease
3. RNA polymerase
4. DNA helicase
1. DNA ligase
2. Restriction endonuclease
3. RNA polymerase
4. DNA helicase
Subtopic: DNA Fingerprinting |
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Why are restriction enzymes critical for DNA fingerprinting?
| 1. | They cut at random points, creating diversity |
| 2. | They amplify DNA |
| 3. | They cut at precise recognition sequences, ensuring reproducibility |
| 4. | They denature DNA for gel loading |
Subtopic: DNA Fingerprinting |
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