| 1. | Prosthetic groups are inorganic, whereas coenzymes are always proteins |
| 2. | Coenzymes remain permanently attached to the apoenzyme, whereas prosthetic groups bind transiently |
| 3. | Prosthetic groups are tightly bound to the apoenzyme, whereas coenzymes usually associate temporarily during catalysis |
| 4. | Coenzymes form the active site permanently, whereas prosthetic groups participate in many unrelated reactions |