Q. No.Why is Taq polymerase used in PCR?
| 1. | It is heat-stable and can withstand the high temperatures of PCR. |
| 2. | It is more accurate than other DNA polymerases. |
| 3. | It can replicate DNA without a template. |
| 4. | It denatures at lower temperatures, speeding up the PCR process. |
Subtopic: Polymerase Chain Reaction: PCR |
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Level 1: 80%+
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The Taq polymerase enzyme is obtained from:
1. Thiobacillus ferrooxidans
2. Bacillius subtilis
3. Pseudomonas putida
4. Thermus aquaticus
2. Bacillius subtilis
3. Pseudomonas putida
4. Thermus aquaticus
Subtopic: Polymerase Chain Reaction: PCR |
95%
Level 1: 80%+
NEET - 2016
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Subtopic: Polymerase Chain Reaction: PCR |
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What is the sequence of steps in PCR?
| 1. | Amplification, insertion, polymerisation |
| 2. | Denaturation, annealing, chain elongation |
| 3. | Annealing, chain termination, denaturation |
| 4. | Annealing, amplification, polymerisations |
Subtopic: Polymerase Chain Reaction: PCR |
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| Assertion (A): | Thermostable polymerase is used in a PCR (Polymerase Chain Reaction). |
| Reason (R): | It remains active at high temperature and causes amplification of DNA during PCR. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A) |
| 2. | Both (A) and (R) are True and (R) is not the correct explanation of (A) |
| 3. | (A) is True, (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Polymerase Chain Reaction: PCR |
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Thermostable DNA polymerase used in PCR was isolated from:
| 1. | Thermus aquaticus | 2. | Escherichia coli |
| 3. | Agrobacterium tumefaciens | 4. | Bacillus thuringiensis |
Subtopic: Polymerase Chain Reaction: PCR |
90%
Level 1: 80%+
NEET - 2023
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Amplification of gene of interest by using PCR for 30 cycles may go upto
1. 0.1 million times
2. 1.0 milllon times
3. 1.0 billion times
4. 1.0 trillion times
1. 0.1 million times
2. 1.0 milllon times
3. 1.0 billion times
4. 1.0 trillion times
Subtopic: Polymerase Chain Reaction: PCR |
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During the process of gene amplification using PCR, very high temperature is not maintained in the beginning, then which of the following PCR will be affected first?
1. Denaturation
2. Ligation
3. Annealing
4. Extension
Subtopic: Polymerase Chain Reaction: PCR |
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Subtopic: Polymerase Chain Reaction: PCR |
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Which of the following has popularized the Polymerase Chain Reaction?
| 1. | Easy availability of DNA template |
| 2. | Availability of synthetic primers |
| 3. | Availability of cheap deoxy ribonucleotides |
| 4. | Availability of ' Thermostable ' DNA polymerase |
Subtopic: Polymerase Chain Reaction: PCR |
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