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Q. No.Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement:
| 1. | The enzyme recognizes a specific palindromic nucleotide sequence in the DNA |
| 2. | The enzyme cuts DNA molecules at an identified position within the DNA |
| 3. | The enzyme binds DNA at specific sites and cuts only one of the two strands |
| 4. | The enzyme cuts the sugar-phosphate backbone at specific sites on each strand |
Subtopic: Restriction Enzymes - Main Enzymes |
77%
Level 2: 60%+
NEET - 2019
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A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using?
| 1. | Eco Rl | 2. | taq polymerase |
| 3. | polymerase lll | 4. | ligase |
Subtopic: Tools: Enzymes: I | Tools |
91%
Level 1: 80%+
NEET - 2016
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Subtopic: Tools: Enzymes: II | Tools |
95%
Level 1: 80%+
NEET - 2016
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Which one of the following statement is not true regarding gel electrophoresis technique?
| 1. | Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light. |
| 2. | The process of extraction of separated DNA strands from gel is called elution. |
| 3. | The separated DNA fragments are stained by using ethidium bromide. |
| 4. | The presence of chromogenic substrate gives blue coloured DNA bands on the gel. |
Subtopic: Separation and Isolation of DNA fragments |
84%
Level 1: 80%+
NEET - 2022
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Biolistics (gene-gun) is suitable for:
| 1. | Transformation of plant cells |
| 2. | Constructing recombinant DNA by joining with vectors |
| 3. | DNA fingerprinting |
| 4. | Disarming pathogen vectors |
Subtopic: Transforming Plant & Animal Cell |
82%
Level 1: 80%+
AIPMT - 2012
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Given below are two statements, one is labelled as Assertion (A) and the other is labelled as Reason (R).
In light of the above statements, choose the correct answer from the options given below:
| Assertion (A): | When a particular restriction enzyme cuts strands of DNA, overhanging stretches or sticky ends are formed. |
| Reason (R): | Some restriction enzymes cut the strand of DNA a little away from the centre of palindromic site. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True and (R) does not correctly explain (A). |
| 3. | (A) is True, (R) is False. |
| 4. | (A) is False, (R) is True. |
Subtopic: Restriction Enzymes - Main Enzymes |
62%
Level 2: 60%+
NEET - 2022
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In genetic engineering, the antibiotic-resistance gene is used:
1. To select healthy vectors
2. As sequences from where replication starts
3. To keep the cultures free of infection
4. As selectable markers
Subtopic: Selectable Markers |
86%
Level 1: 80%+
AIPMT - 2012
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In-gel electrophoresis, separated DNA fragments can be visualized with the help of:
1. Ethidium bromide in UV radiation
2. Acetocarmine in UV radiation
3. Ethidium bromide in infrared radiation
4. Acetocarmine in bright blue light
Subtopic: Separation and Isolation of DNA fragments |
94%
Level 1: 80%+
NEET - 2020
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| Assertion (A): | \(\beta\)-galactosidase coding sequence act as a selectable marker. |
| Reason (R): | Enzyme galactosidase converts galactose into lactose. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are True and (R) is not the correct explanation of (A). |
| 3. | (A) is True but (R) is False. |
| 4. | (A) is False but (R) is True. |
Subtopic: Selection of Recombinant Transformants |
Level 3: 35%-60%
Please attempt this question first.
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Please attempt this question first.
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
| 1. | Polysaccharides | 2. | RNA |
| 3. | DNA | 4. | Histones |
Subtopic: General Design of an rDNA experiment |
89%
Level 1: 80%+
NEET - 2023
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