Which of the following is not required in the preparation of a recombinant DNA molecule?
| 1. | Restriction endonuclease | 2. | DNA ligase |
| 3. | DNA fragments | 4. | E.coli |
| Assertion (A): | A piece of DNA, if needed to be propagated during recombinant DNA procedures, requires a vector. |
| Reason (R): | The vectors provide the origin of replication. |
| 1. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 2. | (A) is False but (R) is True. |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are True and (R) correctly explains (A). |
| I: | Transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through the cell membrane(s). |
| II: | Transformation is one of three processes that lead to horizontal gene transfer, in which exogenous genetic material passes from one bacterium to another. |
| 1. | Transduction | 2. | Conjugation |
| 3. | Transformation | 4. | Translation |