- 1(current)
Q. No.Gel electrophoresis is used for:
| 1. | cutting of DNA into fragments |
| 2. | separation of DNA fragments according to their size |
| 3. | construction of recombinant DNA by joining with cloning vectors |
| 4. | isolation of DNA molecule |
Subtopic: Â Process of Biotech |Â Separation and Isolation of DNA fragments |
 91%
Level 1: 80%+
AIPMT - 2008
Hints
While isolating DNA from bacteria, which of the following enzymes is not required?
1. Lysozyme
2. Ribonuclease
3. Deoxyribonuclease
4. Protease
Subtopic: Â Process of Biotech |Â Obtaining Copy of Gene from Donor DNA |Â Host & Desired DNA |
 75%
Level 2: 60%+
Hints
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
Choose the correct answer from the options given below:
| A. | Insertion of recombinant DNA into the host cell. |
| B. | Cutting of DNA at specific location by restriction enzyme. |
| C. | Isolation of desired DNA fragment. |
| D. | Amplification of gene of interest using PCR. |
| 1. | B, D, A, C | 2. | B, C, D, A |
| 3. | C, A, B, D | 4. | C, B, D, A |
Subtopic: Â Process of Biotech |
Level 3: 35%-60%
NEET - 2023
Hints
Spooling is:
| 1. | Amplification of DNA |
| 2. | Cutting of separated DNA bands from the agarose gel |
| 3. | Transfer of separated DNA fragments to synthetic membranes |
| 4. | Collection of isolated DNA |
Subtopic: Â Process of Biotech |Â Separation and Isolation of DNA fragments |
 73%
Level 2: 60%+
NEET - 2020
Hints
Links
Select the correct statement from the following:
| 1. | Gel electrophoresis is used for the amplification of a DNA segment. |
| 2. | The polymerase enzyme joins the gene of interest and the vector DNA. |
| 3. | Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions. |
| 4. | PCR is used for the isolation and separation of genes of interest. |
Subtopic: Â Process of Biotech |Â Separation and Isolation of DNA fragments |
 74%
Level 2: 60%+
NEET - 2020
Hints
Links
- 1(current)
Q. No.
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