Q. No.Which of the following is most used in genetic engineering:
1. E. coil and Agrobacterium
2. Mycobacteria and Salmonella
3. Aspergillus
4. Penicillium
Which of the following are used in gene cloning:
1. Lomasomes
2. Mesosomes
3. Plasmids
4. Nucleoids
In genetic engineering, a DNA segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector/vectors:
| A. | a bacterium | B. | plasmid |
| C. | plasmodium | D. | bacteriophage |
Options:
| 1. | (A) only | 2. | (A) and (C) only |
| 3. | (B) and (D) only | 4. | (A), (B) and (D) only |
The most important feature in a plasmid to serve as a vector in gene cloning experiment is:
1. Origin of replication (ori)
2. Presence of a selectable marker
3. Presence of sites for restriction endonuclease
4. Its size
| 1. | The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid. |
| 2. | The gene 'X' is 'for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics. |
| 3. | Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance. |
| 4. | The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid. |
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
| List-I | List-II | ||
| A. | Ampicillin | I. | Restriction sites |
| B. | Ori | II. | Blue/White screening |
| C. | Multiple cloning sites | III. | Selectable marker |
| D. | \(\beta\) galactosidase gene | IV. | Sequence from where replication starts |
1. A-III, B-IV, C-I , D-II
2. A-III, B-I, C-IV, D-II
3. A-IV, B-III, C-II , D-I
4. A-II, B-IV, C-I, D-III
| 1. | PsT I | 2. | Pvu I |
| 3. | EcoR I | 4. | BamH I |
| List-I | List-II | ||
| (A) | Kanamycin | (I) | Delivers genes into animal cells |
| (B) | ClaI | (II) | Selectable marker |
| (C) | Disarmed retroviruses | (III) | Restriction site |
| (D) | Kanamycin Rgene | (IV) | Antibiotic resistance |
Choose the correct answer from the options given below:
| Options: | (A) | (B) | (C) | (D) |
| 1. | (II) | (III) | (I) | (IV) |
| 2. | (III) | (I) | (IV) | (II) |
| 3. | (IV) | (III) | (I) | (II) |
| 4. | (II) | (IV) | (I) | (III) |
1. Presence of two or more recognition sites
2. Presence of origin of replication
3. Presence of a marker gene
4. Presence of single restriction enzyme site
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