- 1(current)
Q. No.| 1. | Agrobacterium mediated transformation |
| 2. | Gene gun |
| 3. | 'Disarmed pathogen' vectors |
| 4. | Bacteriophages |
The DNA molecule to which the gene of interest is integrated for cloning is called:
| 1. | Transformer | 2. | Vector |
| 3. | Template | 4. | Carrier |
An enzyme catalysing the removal of nucleotides from ends of DNA is:
| 1. | DNA ligase | 2. | Endonuclease |
| 3. | Exonuclease | 4. | Protease |
2. Palindromic sequence
3. Recognition site
4. Selectable marker
The specific palindromic sequence which is recognized by EcoRI is:
| 1. | 5' -GGAACC-3' 3'-CCTTGG-5' |
2. | 5'-CTTAAG-3' 3'-GAATTC-5' |
| 3. | 5'-GGATCC-3' 3'-CCTAGG-5' |
4. | 5'-GAATTC-3' 3'-CTTAAG-5' |
Which vector can clone only a small fragment of DNA?
1. Bacterial artificial chromosome
2. Yeast artificial chromosome
3. Plasmid
4. Cosmid
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using?
| 1. | Eco Rl | 2. | taq polymerase |
| 3. | polymerase lll | 4. | ligase |
Match the following enzymes with their functions:
|
Column-I |
Column-II |
||
|
(a) |
Restriction endonuclease |
(i) |
joins the DNA fragments |
|
(b) |
Exonuclease |
(ii) |
extends primers on genomic DNA template |
|
(c) |
DNA ligase |
(iii) |
cuts DNA at a specific position |
|
(d) |
Tag polymerase |
(iv) |
removes nucleotides from the ends of DNA |
Select the correct option from the following:
| Options: | (a) | (b) | (c) | (d) |
| 1. | (iii) | (i) | (iv) | (ii) |
| 2. | (iii) | (iv) | (i) | (ii) |
| 3. | (iv) | (iii) | (i) | (ii) |
| 4. | (ii) | (iv) | (i) | (iii) |
Which of the following are used in gene cloning?
1. Lomasomes
2. Mesosomes
3. Plasmids
4. Nucleotides
- 1(current)
Q. No.
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