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Q. No.

Identify a character that is not desirable in a cloning vector:

1.  an inactive promoter
2.  an origin of the replication site
3.  selectable markers such as genes for antibiotic resistance
4.  one or more unique restriction endonuclease sites

Subtopic:  Cloning Vector |
 68%
Level 2: 60%+
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Restriction enzymes are synthesized by:

1.bacteria only2.yeast and bacteria
3.eukaryotic cells only4.all kinds of cells

Subtopic:  Tools: Enzymes: I | Tools: Enzymes: II | Tools: Vectors: II |
 72%
Level 2: 60%+
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The first type II restriction endonuclease discovered that could cut dsDNA at a specific site was:

1. EcoRI 2. SmaI
3. Hind II 4. Hind III
Subtopic:  Tools: Enzymes: I | Tools: Enzymes: II |
 90%
Level 1: 80%+
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A danger of genetic engineering is that the genetically modified bacterium can escape the lab and infect humans. This possibility is prevented by:

1.  selecting a mutant form of the bacteria
2.  selecting a live attenuated bacterium
3.  application of stringent asepsis in the lab
4.  prophylactically immunizing the persons working in the lab
Subtopic:  General Design of an rDNA experiment |
 58%
Level 3: 35%-60%
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A DNA library is:

1.  information in a computer database about all the genes sequenced so far
2.  a collection of vectors in a molecular biology lab
3.  a collection of fragments of DNA that make up the entire genome of an organism
4.  all the restriction sites put together on a DNA fragment
Subtopic:  Introduction & History | Tools |
 66%
Level 2: 60%+
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A foreign DNA was introduced at a cloning site within the gene for beta-galactosidase in a plasmid of a bacterium. The bacterium containing this rDNA was plated on a medium containing X-gal. They were distinguished by the fact that they remained white. This method should be called as:

1.  selection 2.  screening
3.  transformation 4.  transduction
Subtopic:  Selection of Recombinant Transformants |
Level 3: 35%-60%
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Cohen and Boyer constructed one of the first genetically engineered chimeras, pSC101. They introduced in this plasmid the gene of Xenopus laevis that coded for:

1. cytochrome c 2. haemoglobin
3. keratin 4. rRNA

Subtopic:  Restriction Enzymes: Historical Background |
Level 3: 35%-60%
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If the required gene has been isolated by shotgun approach, care must be taken to:

1.  cut the plasmid with a different restriction enzyme than that used to obtain the donor gene
2.  cut the plasmid with the same restriction enzyme that was used to obtain the donor gene
3.  avoid cutting the plasmid
4.  cut the plasmid with two different restriction enzymes
Subtopic:  Selection of Recombinant Transformants |
 85%
Level 1: 80%+
Hints

The chronological steps of a polymerase chain reaction are:

1. Denaturation, Annealing of primers, Primer extension
2. Annealing of primers, Denaturation, Primer extension
3. Annealing of primers, Primer extension, Denaturation
4. Denaturation, Primer extension, Annealing of primers
Subtopic:  Polymerase Chain Reaction: PCR |
 90%
Level 1: 80%+
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Taq polymerase is used in the polymerase chain reaction because:

1. It replicates DNA faster than other enzymes
2. It is the only enzyme that can replicate DNA invitro
3. It does not require an RNA primer to function
4. It is thermostable
Subtopic:  Polymerase Chain Reaction: PCR |
 92%
Level 1: 80%+
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