Q. No.In rDNA technology or genetic engineering elution means :-
1.
Remove the DNA from centrifuge tube after centrifugation
2.
Separation of the recombinant protein from recombinant cell
3.
Insertion of recombinant DNA into host cell
4.
The separated band of DNA are cut out from the gel and extracted from the gel piece
Modern biotechnology consist:-
1. Microbiology
2. Tissue culture
3. Genetic engineering
4. All the above
If a DNA fragment is cut by EcoRI at a specific recognition site then
| 1. | Blunt-end cannot be obtained |
| 2. | Sticky-ends cannot be obtained |
| 3. | Construction of rDNA would not be possible |
| 4. | The plasmid with one site get cut into two fragments |
To transform a bacterial cell with recombinant DNA, which of the following is required?
| 1. | 90°C temperature |
| 2. | Divalent calcium ions |
| 3. | Selectable marker |
| 4. | Restriction enzyme digested DNA |
The construction of the first recombinant DNA by linking antibiotic resistance gene with a native plasmid of Salmonella typhimurium was done by
| 1. | Arber and Nathans |
| 2. | Kary Mullis |
| 3. | Herbert Boyer and Stanley Cohen |
| 4. | Smith and Wilcox |
'Insertional inactivation' is used for
| 1. | Selection of recombinants |
| 2. | Producing pest resistant crops |
| 3. | Enhancing nutritional value of food |
| 4. | Reducing post harvest losses |
Given below are the three basic steps in genetically modifying an organism
| a. | Introduction of the identified DNA into the host. |
| b. | Maintenance of introduced DNA in the host and transfer of the DNA to its progeny. |
| c. | Identification of DNA with desirable genes. |
Choose the option with respect to correct sequence of the given steps
1. a → c → b
2. c → b → a
3. c → a → b
4. b → a → c
Method of gene transfer that involves use of electrical impulses of high field strength to increase permeability of membrane by creating transient pores in it, is
| 1. | Microinjection |
| 2. | Electroporation |
| 3. | Biolistics |
| 4. | Lipofection |
The figure below shows three steps (A, B, C) of polymersase chain reaction (PCR). Select the correct identification together with what it represents
1. B - Denaturation at a temperature of about 98°C separating the two DNA strands
2. A - Denaturation at a temperature of about 60°C
3. C - Extension in the presence of heat stable DNA polymerase
4. A - Annealing with two sets of primer
Which of the following statements is incorrect with respect to polymerase chain reaction?
| 1. | Repeated amplification is achieved by the use of thermostable DNA polymerase |
| 2. | Thermostable DNA polymerase is isolated from a bacterium, Thermus aquaticus |
| 3. | Each cycle in PCR has three steps, first is primer annealing, second is denaturation and third is extension of primers |
| 4. | In polymerase chain reaction, multiple copies of gene of interest are synthesized in vitro using two sets of primers and enzyme DNA polymerase |
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