(a) List the two methodologies which were involved in human genome project. Mention how they were used.
(b) Expand 'YAC' and mention what was it used for. (3)
(a) Expressed Sequence Tags , Identifying all the genes that are expressed as RNA = ½ + ½ Sequence Annotation , sequencing the whole set of genome coding or non coding sequences and later assigning different region with functions = ½ + ½
(b) Yeast Artificial Chromosome , used as cloning vectors (cloning / amplification ) = ½ + ½