Restriction enzyme EcoRI cuts

A.      Between bases G and A

B.      Cuts between GAATTC

C.      Cuts between G and A  at GAATTC

D.     Both A and C

Concept Questions :-

Tools: Enzymes:II
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Restriction endonuclease cuts

A.      Exactly at the centre of site

B.      Cutting of both the strands occur one after another

C.      Between the same to bases on the opposite strands

D.     Both A and C

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EcoRI cuts

A.      A little away from the centre

B.      Both the strands at the same time

C.      Creates blunt ends

D.     Both A and B

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DNA gel electrophoresis is commonly prepared by

A.      Agarose, a natural polymer

B.      Extracted from sea weeds

C.      A synthetic polymer

D.     Both A and B

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Which of the following step is first one in order to separate DNA fragments?

A.      Running of gel

B.      Staining of gel

C.      Elution

D.     Setting of gel

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Which of the following step is last for isolating the desired DNA fragment?

A.      Running of gel

B.      Elution

C.      Staining

D.     Visualization

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Arrange the following steps in order:

1) Restriction Digestion 2) Running Agarose gel 3) Elution of bands 4) Purification of DNA 5)ligation

6) transformation 7) Cloning

A.      1-2-3-4-5-6-7

B.      1-5-6-7-2-3-4

C.      1-2-3-5-6-7-4

D.     2-3-4-1-5-6-7

Concept Questions :-

Intro & History: II
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Which of the following steps will be performed after restriction digestion?

A.      Ligation

B.      Elution from DNA gel

C.      Transformation

D.     Cloning

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If one performs an experiment of restriction digestion and then without separating bands from DNA goes for ligation reaction. There will be

A.      Non specific ligations

B.      Undesired products

C.      No ligation

D.     Both A And B

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In a DNA gel the fragment of 2kb and 4kb will be

A.      2kb nearer to cathode while 4kb nearer to anode

B.      2kb closer to wells while 3kb towards the opposite ends

C.      2kb closer to opposite end of gel while 3kb closer to well

D.     2kb away from cathode while 3kb towards anode

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