Which of the following is a recombinant protein-

  1. b Galactosidase in E.coli
  2. Insulin in humans
  3. Somatotropin in humans
  4. Interferon in E. coli

Concept Questions :-

Large Scale Production: II
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An example of a transgenic is

  1. E.coli expressing b Galactosidase gene
  2. Carrot expressing Nif genes
  3. HIV expressing Reverse transcriptase gene
  4. Brassica expressing erusic acid gene
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Which of the following would you choose as the safest and least cumbersome selectable marker-

  1. Ampicillin resistance gene
  2. Tetracyclin resistance gene
  3. Kanamycin resistance gene
  4. b Galactosidase gene

Concept Questions :-

Selectable Markers:III
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Recombinant DNA is formed in nature during-

  1. Crossing over at pachytene
  2. Integration of plasmid to form episome
  3. Integration of phage DNA to form prophage
  4. More than one is correct.
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Southern Blotting essentially makes use of a

  1. Buffer
  2. Electrodes
  3. Agarose gel
  4. All of the above
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The native charge on DNA molecules is

  1. Positive
  2. Negative
  3. Neutral
  4. DNA is an ampholyte
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The visualization of the DNA bands on the gel can be done with the help of

  1. UV rays
  2. Ethylene bromide
  3. Chromogenic substrate
  4. More than one is correct
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In electrophoresis, the DNA fragments to be separated are loaded into wells on the

  1. Anode end
  2. Cathode end
  3. Either cathode or anode depending on the treatment of DNA
  4. Neutral end

Concept Questions :-

Process of Biotech
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If one needs to derive a cDNA of human insulin, which cells should be taken up for extraction?

  1. Any cell of the human body as the DNA is same in all cells
  2. Any nucleated cell of the human body
  3. b cells of islets of Langerhans of human pancreas
  4. WBCs

Concept Questions :-

Host & Desired DNA: II
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A transgenic is an organism which

  1. Incorporates the foreign gene
  2. Incorporates and expresses the foreign gen
  3. Incorporates, expresses and passes the foreign gene to the next generation.
  4. Incorporates, expresses, inherits and mutates a foreign gene.

Concept Questions :-

Intro & History: II
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