Restriction enzymes do not act on the the DNA of the Host cell in which they are produced because
a. Host DNA is packed into chromosomes
b. Restriction enzymes are ineffective on host DNA
c. Host DNA does not have the restriction site for the Restriction enzymes.
d. Restriction site of host DNA is methylated.

Concept Questions :-

Tools: Enzymes:II
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A protein is not expressed properly in a diseased tissue. To find out whether the defect is at the level of translation or post translational modifications which techniques would you use ?
a. Southern blotting and South Western blotting
b. Northern blotting and western blotting
c. Western blotting and Eastern blotting
d. Southern and Northern blotting

Concept Questions :-

Process of Biotech
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Insertional inactivation of a gene helps in
a. Identification of recombinant clones
b. Identification of deletion mutants
c. Identification of recombinant transformants
d. Elimination of suppression mutants

Concept Questions :-

Selectable Markers:III
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What is true about the DNA polymerase used in PCR?

  1. Isolated from a virus
  2. Stable at high temperatures
  3. Used as selectable marker
  4. Has methylated bases
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Biolistic method makes use of microparticles coated with DNA bombarded at cells to be transformed. These particle are made up of
a. Zinc or tungsten
b. Silicon or gold
c. Tungsten or gold
d. Selenium or Platinum

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Look at the given hypothetical vector-

Suppose we cut the desired gene as well as the plasmid with the help of the Restriction enzyme EcoR I and ligate them. What would be true-

  1. Kanamycin resistance will select the transformants from non transformants while tetracycline resistance will select recombinant transformants from non-recombinant
  2. Tetracyclin resistance will select the transformants from non transformants while kanamycin resistance will select recombinant transformants from non-recombinant 
  3. Both will select only transformants.
  4. Both will select only recombinants.

Concept Questions :-

Tools: Vectors:II
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Insertional inactivation of the lac Z gene forms-

  1. Blue recombinant colonies
  2. Colourless recombinant colonies
  3. Fluorescent green colonies
  4. There is no relation between the lac Z gene and colour of the colony.

Concept Questions :-

Selectable Markers:III
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A reporter enzyme used in biotechnology is-

  1. Green Fluorescent Protein
  2. Luciferase
  3. b Galactosidase
  4. All of the above
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Downstream processing does not include

  1. Quality control
  2. Clinical trials
  3. Product purification
  4. Sampling from bioreactor

Concept Questions :-

Large Scale Production: II
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Probes are molecules of

  1. DNA
  2. RNA
  3. Proteins
  4. All of the above

Concept Questions :-

Process of Biotech
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