Somaclonal variation can be obtained by
(a) application of colchicine
(b) irradiation with gamma rays
(c) tissue culture
(c) Somaclonal variation refers to all types of variation occur in plants regenerated from cultured cells or tissues. Genetic changes take place in plant tissue culture and these changes take place in plant tissue culture and these changes are passed to their progenies. This technology can be used for disease resistance, improvement of quality and yield in plants.