Target DPP Test

1.

The cutting of DNA at specific locations became possible with the discovery of?
1. Restriction enzymes
2. Probes
3. Selectable markets
4. ligases

2.

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:
1. Polymerase chain reaction
2. Electrophoresis
3. Restriction mapping
4. Centrifugation

3.

Restriction endonucleases are enzymes which:

1.	make cuts at specific positions within the DNA molecule
2.	recognize a specific nucleotide sequence for binding of DNA ligase
3.	restrict the action of the enzyme DNA polymerase
4.	remove nucleotides from the ends of the DNA molecule

4.

Refer to this sketch of a gel with four samples of DNA. Which well contained DNA that did not undergo restriction digestion and which lane contains the smallest piece of DNA?

1.	3 & 2	2.	2 & 3
3.	3 & 4	4.	2 & 1

5.

Select the correct statement .

1.	A restriction exonuclease cuts both strands of foreign DNA as well as vector DNA at specific palindromic sequences
2.	A restriction endonuclease is named on the basis of scientific name and strain of bacteria from which it is isolated
3.	All restriction enzymes cut the strands of DNA in the centre of palindromic site between the same two bases of opposite strand
4.	When cut by same restriction enzyme, the resultant DNA fragments generate different kinds of sticky ends

6.

Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?
1. Plant cells - Cellulase
2. Algae - Methylase
3. Fungi - Chitinase
4. Bacteria – Lysozyme

7.

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
1. Ligase
2. Eco RI
3. Taq polymerase
4. polymerase II

8.

Which one of the following represents a palindromic sequence in DNA?
1. 5' - GAATTC - 3'
3' - CTTAAG - 5'
2. 5' - CCAATG - 3'
3' - GAATCC - 5'
3. 5' - CATTAG - 3'
3' - GATAAC - 5'
4. 5' - GATACC - 3'
3' - CCTAAG - 5'

9.

DNA fragments separated by gel electrophoresis are shown. Mark the correct statement :

1.	Band 3 contains more positively charged DNA molecules than band 1.
2.	Band 3 indicates more charge density than bands 1 and 2.
3.	Band 1 has longer DNA fragment than bands 2 and 3.
4.	All bands have equal lengths and charges but differ in base composition.

10.

Which of the following is incorrect about restriction endonuclease enzyme?
(1) It cuts strands of DNA at specific points in their sugar phosphate backbones
(2) It recognizes palindromic nucleotide sequences in the DNA
(3) It belongs to a class of enzymes called nucleases
(4) It removes nucleotides from the ends of DNA

11.

To isolate DNA in pure form from a bacterial cell, it should initially be treated with:

1.	Proteases and RNase but not DNase	2.	Lysozyme
3.	Restriction endonuclease	4.	DNA ligase

12.

Restriction endonucleases:

1.	Are used in genetic engineering for ligating two DNA molecules.
2.	Are used for in vitro DNA synthesis.
3.	Are synthesized by bacteria as part of their defense mechanism.
4.	Are present in mammalian cells for the degradation of DNA when the cell dies.

13.

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:

1.	Palindromic Nucleotide sequences	2.	Poly(A) tail sequences
3.	Degenerate primer sequence	4.	Okazaki sequences

14.

Assertion (A):	In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes.
Reason (R):	Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning.

1.	Both (A) and (R) are True and the (R) is the correct explanation of (A)
2.	Both (A) and (R) are True but the (R) is not the correct explanation of (A)
3.	(A) is True but (R) is False
4.	Both (A) and (R) are False

15.

Given below are four statements pertaining to the separation of DNA fragments using Gel electrophoresis. Identify the incorrect statements:

(a)	DNA is a negatively charged molecule and so it is loaded on gel towards the Anode terminal.
(b)	DNA fragments travel along the surface of the gel whose concentration does not affect the movement of DNA.
(c)	Smaller the size of the DNA fragment, the larger is the distance it travels through it.
(d)	Pure DNA can be visualized directly by exposing to UV radiation.

1.	(a), (c) and (d)
2.	(a), (b) and (c)
3.	(b), (c) and (d)
4.	(a), (b) and (d)

16. The DNA sequence shown below is:
5’ – GAATTC – 3’
3’ – CTTAAG – 5’

1.	the consensus sequence for prokaryotic promoter
2.	the most common VNTR in the human genome
3.	the recognition sequence of a common restriction endonuclease EcoR I
4.	an intron within the eukaryotic split gene

17.

Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R)

Assertion (A):	Restriction enzyme is a type of endonuclease
Reason (R):	Restriction enzyme cuts the two stands of DNA at specific positions within the DNA

In the light of the above statements, choose the correct answer from the options given below:

1.	(A) is True but (R) is False.
2.	(A) is False but (R) is True.
3.	Both (A) and (R) are True and (R) is the correct explanation of (A).
4.	Both (A) and (R) are True but (R) is not the correct explanation of (A).

18. Identify the palindromic sequence in the following
1. \({{GAATTC}\over{GAATTC}}\)
2. \({{GAATTC}\over{CTTUUG}}\)
3. \({{GAATTC}\over{CUUAAG}}\)
4. \({{GAATTC}\over{CTTAAG}}\)

19. Given below are two statements, one is labelled as Assertion (A) and the other is labelled as Reason (R).

Assertion (A):	When a particular restriction enzyme cuts strands of DNA, overhanging stretches or sticky ends are formed.
Reason (R):	Some restriction enzymes cut the strand of DNA a little away from the centre of palindromic site.

In light of the above statements, choose the correct answer from the options given below:

1.	Both (A) and (R) are True and (R) correctly explains (A).
2.	Both (A) and (R) are True and (R) does not correctly explain (A).
3.	(A) is True, (R) is False.
4.	(A) is False, (R) is True.

20. Separation of DNA, fragments is done by a technique known as :
1. Polymerase Chain Reaction
2. Recombinant technology
3. Southern blotting
4. Gel electrophoresis

21.

Assertion(A):	The restriction endonucleases can recognize palindromic sequences.
Reason(R):	These sequences read the same in both directions of the two complementary DNA strands.

1.	Both (A) and (R) are True and (R) is the correct explanation of (A).
2.	Both (A) and (R) are True but (R) is not the correct explanation of (A).
3.	(A) is True but (R) is False.
4.	Both (A) and (R) are False.

22.

Assertion(A):	Action of restriction enzyme on a DNA fragment may create sticky ends.
Reason(R):	The stickiness of the ends facilitates the action of the enzyme DNA ligase.

1.	Both (A) and (R) are True and (R) is the correct explanation of (A).
2.	Both (A) and (R) are True but (R) is not the correct explanation of (A).
3.	(A) is True but (R) is False.
4.	Both (A) and (R) are False.

23. Which of the following pairs is correctly matched?
1. Central dogma — Codon
2. Okazaki fragments — Splicing '
3. RNA polymerase —-RNA primer
4. Restriction enzymes — Genetic engineering

24. The figure below is the diagrammatic representation of a typical agarose gel electrophoresis.

Choose the correct option.

1.	DNA fragments move towards anode because they are positively charged
2.	Separation of DNA fragments occurs due to sieving effect in gel
3.	Larger DNA fragment move faster than smaller fragments
4.	DNA fragments are visualized after staining with bromoethane in green light

25. Consider the given two statements:

Assertion (A):	In agar gel electrophoresis, DNA moves towards the positive electrode (anode).
Reason (R):	DNA is a hydrophilic molecule.

1.	Both (A) and (R) are True and (R) correctly explains (A).
2.	(A) is True; (R) is False
3.	Both (A) and (R) are False
4.	Both (A) and (R) are True but (R) does not correctly explain (A)

26. The common restriction enzyme EcoRI recognizes the palindromic sequence:
1. GAATTC
2. CCCGGG
3. AAGCTT
4. TGGCCA

27. Consider the given two statements:

Assertion (A):	Restriction enzymes are found in bacteria and provide a defense mechanism against invading viruses.
Reason (R):	They interact with membranes of the host bacterial cells and remove all the receptor sites by the viruses.

1.	Both (A) and (R) are True and (R) correctly explains (A).
2.	Both (A) and (R) are True but (R) does not correctly explain (A).
3.	(A) is True; (R) is False
4.	Both (A) and (R) are False

28. The restriction endonuclease enzymes have been isolated from:

1.	Bacteria	2.	Yeasts
3.	Algae	4.	Fungi

29. The restriction endonuclease enzymes used in rDNA technology cut:
1. ssDNA
2. dsDNA
3. ssRNA
4. dsRNA

30. To isolate DNA in pure form from a plant cell, the plant cell must first be treated with:
1. Ice
2. Calcium chloride
3. Cellulase
4. Lysozyme

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