What is incorrect about 1 antitrypsin?
(1) It is a lung protecting glycoprotein produced by hepatocytes.
(2) Chronic pulmonary obstructive disease (COPD), bronchitis and emphysema.
(3) A genetic disorder generally leads to its deficiency.
(4) milk enriched with this protein.
The ADA gene is inserted into the target cells using a retroviral vector. What is true about the gene?
(1) It is normally expressed in the bone marrow cells.
(2) It is delivered as a cDNA into the host cells.
(3) It is delivered by the in vivo technique.
(4) The host cells are preferably the Iymphocytes.
A gene silencing technique that uses a ds RNA for suppression of gene expression is
(1) Antisense RNA technique
(2) Interference RNA technique
(3) Heterogenous RNA technique
(4) All of the above
A GMO is different from a hybrid in that it
(1) Is a result of a hybridization between unrelated organisms.
(2) It has only desirable genes from the source organism.
(3) It is selected out through physical examination of various phenotypes.
(4) Involves cross breeding of taxonomically related organisms.
A therapeutic application of Recombinant DNA technology is
(1) Gene therapy
(3) DNA chips or microarrays
(4) DNA hybridization
The second amendment to the Indian Patents Bill does not include
(1) Patents terms
(2) Emergency provisions
(3) Quick redressal of patents lawsuits.
(4) Research initiatives
An argument that defies the patenting of Basmati rice by an American company is
(1) 200000 varieties of rice are found in India.
(2) There are 27 documented varieties of rice grown in India.
(3) There is reference to the Basmati rice in ancient Indian folklore and poetry proving that it has been grown here for centuries.
(4) All of the above
Mark the incorrect statement.
(1) DNA vaccines are segments of genetically engineered DNA of the pathogens that code for antigen.
(2)antigens (proteins) produced by a heterologous host like bacteria.
(3) Example of Recombinant vaccine is Hepatitis B vaccine produced by yeast cells.
(4) In Recombinant vaccines the antigen protein is expressed by the human cells.
Which, in your opinion, is the most long term option for dealing with SCID?
1. ADE Enzyme replacement through injections.
2. Bone marrow replacement
3. Normal ADA gene insertion into the lymphocytes.
4. Normal ADA gene insertion into the bone marrow cells.
Over 95% of all transgenic animals are mice. What do you think is the reason?
1. The organization of their DNA and the wy they express their genes is very similar to humans,
2. They suffer from many similar disease as human like diabetes, cancer and anxiety.
3. Their reproductive and nervous system are very similar to humans.
4. All of the above
Mark the incorrect statement w.r.t. Bt
1. Bt crops are resistant to Lepidopterans, dipterans, and coleopterans, and some arachnids.
2. Bacillus thuringiensis is a soil-dwelling bacterium that produces the protein protoxin.
3. Some insects may become resistant to Bt toxin over time
4. The protoxin is activated in high pH to form active toxin crystal.
The Nobel Prize in medicine in 2006 was awarded to Andrew Fire and Craig C. Mellow for their work in RNA interference on
1. Meliodogyne incognita
2. Caenorhabtidis elegans
3. Arabidopsis thaliana
4. Anguiun tritici
The first crop plant to have its genome sequenced is
A person suffering from night blindness should be advised to include the following in his diet.
1. High lysine corn
2. Golden rice
3. Flavr Savr Tomato
4. Hirudin variety of Brassica
Eukaryotic expression systems are being used to obtain
The first genetically engineered crop to be granted a license for human consumption was Flavr Savr Tomato. It is a variety of tomato in which a gene for Antisense RNA was introduced to inhibit the formation of the enzyme polygalacturonase. This enzyme acts on
Mustard usually has a high erucic acid content of over 40%. Erucic acid is a 22C monounsaturated fatty acid that may pose a health risk. Low erucic transgenic mustard has been developed by blocking the expression of the gene Fatty Acid Elongation 1 (FAE 1). The technique used is likely
1. Antisense RNA technology
2. Interference RNA technology
3. Restriction modification
4. Sense RNA technology
Interference RNA carries out suppression of gene expression at the level of
1. Transcription of mRNA
2. Post transcriptional modifications
3. Transport of mRNA
4. Translation of mRNA
ELISA is not for the detection and quantification of
A circular and linear DNA of the same molecular weight was cut by a restriction enzyme for which there was only one restriction site in each. When DNA fragments were separated by electrophoresis the following observation would be most likely made for circular and linear DNA respectively
1. TWO DNA bands of the same mol. Weight on each gel
2. One heavier DNA band, and Two lighter DNA bands
3. One lighter DNA band and Two heavier DNA bands
4. Two heavier DNA bands and One lighter DNA band
In a landmark decision The European Patents Office recently(2018) revoked the patent awarded to US department of agriculture in 199. This pertained to
1. Antfungal agents of Neem
2. Sweetener of Brazzein
3. Varieties of Basmati
4. Alkaloids of Turmeric
Biopiracy involves illegal appropriation of Natural resources that include
2. Plants and animals
4. All of these
What is incorrect about Cry gene?
1. Mostly encoded by a plasmid gene
2. Produces delta endotoxins
3. Derives its name from the crystal toxins it produces
4. There is a single type of gene producing one type of toxin
A collection of techniques that aim at applying molecular biology to medical testing is called
1. Gene therapy
2. Recombination protein Synthesis
3. Molecular diagnosis
4. Gene silencing
Which of the following would not involve the formation of a recombinant protein in a heterologous host?
1. Humulin production in E.coil.
2. Human lactalbumin in cows.
3. Interferons in bacteria
4. ADA in humans