Home Work #2 - Biotechnology: Principles and ProcessesContact Number: 8527521718Home Work #2 - Biotechnology: Principles and ProcessesContact Number: 8527521718Using recombinant DNA technology, genes from a donor cell can be implanted into a bacterium for DNA replication and protein synthesis. The kind of cell(s) that can be used as gene donors in this technology is/are:
1. Bacteria only
2. Either yeast or bacteria only
3. Eukaryotic cells only
4. Any kind of cell
A gene carried by recombinant DNA is cloned when:
1. Its host bacterium divides by binary fission
2. It is transcribed
3. It is fragmented by restriction enzymes
4. It is hybridized
Which of the following would NOT be a useful selectable marker?
| 1. | A gene encoding a protein that degrades the antibiotic ampicillin. |
| 2. | A gene encoding a protein that allows the cell to synthesize histidine. |
| 3. | A gene encoding a protein that is an essential structural component of the cell. |
| 4. | All of these are useful selectable markers. |
Identify a character that is not desirable in a cloning vector:
| 1. | an inactive promoter |
| 2. | an origin of the replication site |
| 3. | selectable markers such as genes for antibiotic resistance |
| 4. | one or more unique restriction endonuclease sites |
Elution is:
| 1. | separating the restricted DNA fragments on agarose gel |
| 2. | staining the separated DNA fragments with ethidium bromide |
| 3. | cutting out of the separated bands of DNA from the agarose gel and extracting them from the gel piece |
| 4. | constructing rDNA by joining the purified DNA fragments to the cloning vector |
When isolating the pure DNA from a bacterial cell, the cell should not be treated with:
| 1. | lysozyme | 2. | proteases |
| 3. | ribonuclease | 4. | deoxyribonuclease |
Which of the following is not a plasmid?
| 1. | pSC101 | 2. | pUC18 |
| 3. | BamHI | 4. | pBR322 |
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
| 1. | DNA can be seen in visible light |
| 2. | DNA can be seen without staining in visible light |
| 3. | Ethidium bromide stained DNA can be seen in visible light |
| 4. | Ethidium bromide stained DNA can be seen under exposure to UV light |
In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
| 1. | Charge only | 2. | Size only |
| 3. | Charge-to-size ratio | 4. | All of the above |
The most important feature in a plasmid to serve as a vector in gene cloning experiment is:
| 1. | Origin of replication (ori) |
| 2. | Presence of a selectable marker |
| 3. | Presence of sites for restriction endonuclease |
| 4. | Its size |
An antibiotic resistance gene in a vector usually helps in the selection of:
1. Competent bacterial cells
2. Transformed bacterial cells
3. Recombinant bacterial cells
4. None of the above
For the multiplication of any alien piece of DNA in an organism, it needs to be part of a chromosome that has a specific:
| 1. | Telomeric sequence | 2. | Multiple cloning sites |
| 3. | Ori | 4. | Selectable marker |
Which of the following cannot be used as a vector in rDNA technology?
| 1. | Plasmid | 2. | Phage DNA |
| 3. | Bacterium | 4. | YAC |
Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?
| 1. | Plastid | 2. | Bacteriophages |
| 3. | BAC | 4. | Plasmid |
pBR322 does not contain the site for:
| 1. | Pvu I | 2. | BamH I |
| 3. | Sma I | 4. | EcoR I |
Lysozyme should be used when isolating DNA in a pure form from:
| 1. | A bacterial cell | 2. | A fungal cell |
| 3. | A plant cell | 4. | An animal cell |
In recombinant DNA experiments, a vector:
| 1. | carries DNA into a new cell |
| 2. | links together newly joined fragments of DN |
| 3. | makes millions of copies of a specific segment of DNA |
| 4. | separates fragments of DNA by their length and electrical charges |
A piece of DNA, somehow transferred into an alien organism, will be able to multiply itself in the progeny cells of organism if:
| 1. | It includes the centromere |
| 2. | It becomes a part of chromosome |
| 3. | It does not contain any intron sequences |
| 4. | It is a single stranded polynucleotide |
The construction of first rDNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of:
| 1. | Salmonella typhimurium | 2. | Escherechia coli |
| 3. | Agrobacterium tumefaciens | 4. | Haemophilus influenzae |
What controls the copy number of linked DNA if plasmid is used as a vector in bacterial transformation?
| 1. | Ori | 2. | rop |
| 3. | Selectable markers | 4. | Promoter in the insert |
In the given diagram of pBR322, the location of the recognition sequences of BamH I and Sal I are represented by the numbers:
| 1. | 1 and 2 | 2. | 3 and 4 |
| 3. | 6 and 7 | 4. | 5 and 8 |
If you want to isolate DNA in pure form from a fungal cell, the first enzyme required normally would be:
| 1. | Restriction endonuclease | 2. | DNA ligase |
| 3. | DNA polymerase | 4. | Chitinase |