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When the phage DNA enters a bacterium, it protects itself from the viral DNA with the help of:

The enzyme used in the polymerase chain reaction is a:
1. DNA dependent RNA polymerase
2. RNA dependent DNA polymerase
3. DNA dependent DNA polymerase
4. RNA dependent RNA polymerase

An important limitation of the use of Agrobacterium tumefaciens is that it cannot:

Identify a character that is not desirable in a cloning vector:

Restriction enzymes are synthesized by:

A cloning vector has two antibiotic resistance genes- for tetracycline and ampicillin. A foreign DNA was inserted into the tetracycline gene. Non-recombinants transformants would survive on the medium containing:
1. ampicillin but not tetracycline
2. tetracycline but not ampicillin
3. both tetracycline and ampicillin
4. neither tetracycline nor ampicillin

The first type II restriction endonuclease discovered that could cut dsDNA at a specific site was:

Using reverse transcriptase or gene synthesis has an advantage over the shotgun approach in obtaining a copy of the gene required as:

Elution is:

When isolating the pure DNA from a bacterial cell, the cell should not be treated with:

A host cell normally does not take up a foreign DNA until it has been made competent to do so. This is because:

How does the bacterium protects its own DNA from the action of restriction endonucleases present in its cytoplasm?

When “sticky ends” are paired, they can be joined by:

A danger of genetic engineering is that the genetically modified bacterium can escape the lab and infect humans. This possibility is prevented by:

A DNA library is:

A foreign DNA was introduced at a cloning site within the gene for beta-galactosidase in a plasmid of a bacterium. The bacterium containing this rDNA was plated on a medium containing X-gal. They were distinguished by the fact that they remained white. This method should be called as:

Cohen and Boyer constructed one of the first genetically engineered chimeras, pSC101. They introduced in this plasmid the gene of Xenopus laevis that coded for:

Which of the following is not a plasmid?

Identify the incorrectly matched pair:
   restriction enzyme                   recognition site
    1. EcoRI                                5’GAATTC3’
                                                3’CTTAAG5’
     2. HindII                               5’GTPyPuAC3’
                                                3’CAPuPyTG3’
     3. SmaI                                5’GGATTC3’
                                                3’CTTAGG5’
    4. HindIII                              5’AAGCTT3’
                                                3’TTCGAA5’

Which of the following restriction enzymes would not produce “sticky ends”?

If the required gene has been isolated by shotgun approach, care must be taken to:

The chronological steps of a polymerase chain reaction are:

Taq polymerase is used in the polymerase chain reaction because:

A major advantage of using YAC as a cloning vector over the plasmids is that:
1. it can replicate independently
2. it can be selected easily
3. it can accommodate larger inserts
4. it has multiple cloning sites

Which of the following is normally not used as a method of introduction of foreign DNA into an animal cell?