Which of the following process is related to reverse transcription?
1. DNA dependent DNA synthesis
2. RNA dependent DNA synthesis
3. DNA dependent RNA synthesis
4. RNA dependentv polypeptide synthesis
Which of the following structires are present in core particle of nucleosome?
1. Octamer of histone proteins
2. 200 bp of DNA
3. Non-histone proteins
4. Linker DNA
Which of the following radioactive isotopes were utilised for labelling protein and DNA in transduction experiment respectively?
Unwinding of DNA creates tension which is related by enzyme
Out of two strands of DNA one is carrying genetic information for transcription and it is called
1. Coding strand
2. Non template strand
3. Sense starnd
4. Template strand
Poly A tail is present in
1. mRNA of bacteria
2. tRNA of eukaryotes
3. Promotor of bacteria
4. mRNA of eukaryotes
In eukaryotes, RNA polymerase III catalyses the synthesis of
1. 5 S rRNA, tRNA & SnRNA
2. mRNA, HnRNA & SnRNA
3. 28 S rRNA, 18 S rRNA & 5 S rRNA
4. All types of rRNA & tRNA
In lac operon, the lac mRNA
1. Has several initiation and termination codons
2. Forms four different enzymes
3. Is not transcribed in the presence of lactose
4. Is involved in an anabolic reaction
In the technique of DNA fingerprinting digestion of DNA is followed by
4. Southern blotting
If the sequence of one strand of DNA is 5' A T G C A T C G 3', find the sequence of the complementary strand in 5' 3' direction
1. T A C G T A G C
2. C G A T G C A T
3. A T G C A T C G
4. A T C G T A C G
NHC structure proteins are
1. Basic proteins rich in lysine, arginine
2. Regulatory proteins
3. Catalytic proteins rich in tryptophan and arginine
4. Required for packaging of chromatin at higher levels.
Which of the following bond is not present in DNA?
1. glycosidic bond
2. phosphodiester bond
3. glycosidic bond
4. glycosidic bond
How many trypes of DNA polymerases are present in bacteria?
For the strand seperation and stabilisation during DNA replication which of the following set of enzymes and proteins are required?
1. SSBP, gyrase, primase
2. Topoisomerase, helicase, ligase
3. Gyrase, ligase, primase
4. Topoisomerase, helicase, SSBP
What is correct for bacterial transcription?
1. mRNA requires processing to become active
2. Translation can begin when mRNA is fully transcribed
3. Transcription and translation takes place in the same compartment
4. Rho factor initiates the process
In-vitro template independent RNA synthesis is a feature of
1. RNA polymerase
2. Reverse transcriptase
3. Ochoa enzyme
4. DNA polymerase
In protein synthesis, which of the following are required for the synthesis of charged tRNA?
1. Amino acid, GTP, initiation codon, ribosome
2. Amino acid, ATP, , enzyme, tRNA
3. Amino acid, ATP, , enzyme, mRNA
4. Aminoacyl tRNA, ribosome, initiation codon, release factor
The accessibility of promoter regions of bacterial DNA in many cases regulated by the interaction of proteins with sequences termed
2. structural genes
3. Inhibitor genes
Select the correct one (w.r.t. wobble hypothesis)
1. Third base of a codon lacks vibrating capacity
2. Third base can establish H-bonds even with the non-complementary anticodon
3. Specificity of a anticodon is particularly determined by first two codon
4. Major cause of degeneracy is the first two N-bases of codon
If there are 81 million bases in RNA of human cell, then calculate the total number of introns present in cDNA
1. 27 millions
3. Equal to ribonucleotides
4. Half the number of ribonucleotides
Majority of unusual bases are found in tRNA, loop is
1. First loop from 5' - end of tRNA
2. AA - tRNA synthetase binding loop
3. Ribosomal binding loop
4. Nodoc site
How many amino acids will be coded by the mRNA sequence - 5'CCCUCAUAGUCAUAC3' if a adenosine residue is inserted after 12th nucleotide?
1. Five amino acids
2. Six amino acids
3. Two amino acids
4. Three amino acids
Identification and binding of RNA polymerase to the promoter sequence is a function of
1. Rho factor
2. Sigma factor
3. Beta factor
4. Omega factor
Pribnow box is a consensus of ____________ bases, forming a binding site for E. coli RNA polymerase at promotor.
When the genomes of two people are cut using the same restriction enzyme, the length and number of fragments obtained are different, this is called
4. Northern blotting
Which of the following does not code for any proteins?
4. More than one option is correct
In which step of DNA profiling, nitrocellulose membrane is used?
4. DNA amplification
All of the following are part of an operon except
1. an operator
2. structural genes
3. a promoter
4. an enhancer
AGGTATCGCAT is a sequence from the coding strand of a gene. What will be the corresponding sequence of the transcribed mRNA?
Select the correct match
1. Alec Jeffreys - Streptococcus pneumoniae
2. Alfred Hershey and Martha Chase - TMV
3. Francois Jacob and Jacques Monod - Lac operon
4. Matthew Meselson and F. Stahl - Pisum sativum
The final proof for DNA as the genetic material came from the experiments of
2. Hershey and Chase
3. Avery, Mcleod and McCarty
4. Hargobind Khorana
DNA replication in bacteria occurs
1. During S-phase
2. Within nucleolus
3. Prior to fission
4. Just before transcription
Which of the following RNAs should be most abundant in animal cell?
During DNA replication, Okazaki fragments are used to elongate
1. The leading strand towards replication fork
2. The lagging strand towards replication fork
3. The leading strand away from replication fork
4. The lagging strand away from the replication fork
Taylor conducted the experiments to prove semiconservative mode of chromosome replication on
1. Vince rosea
2. Visia faba
3. Drosophila melanogaster
4. E. coli
The equivalent of a structural gene is
Which of the following rRNAs acts as structural RNA sa well as ribozyme in bacterial?
1. 5 S rRNA
2. 18 s rRNA
3. 23 s rRNA
4. 5.8 S rRNA
Which of the following is required as inducer(s) for the expression of Lac operon?
1. Lactose and Galactose
Which of the following is not required for any of the techniques of DNA fingerprinting available at present?
1. DNA - DNA hybridization
2. Polymerase chain reaction
3. Zinc finger analysis
4. Restriction enzymes
Satellite DNA is important because it
1.code for enzymes needed for DNA replication
2. Codes for proteins needed in cell cycle
3. Shows high degree of polymorphism in population and also the same degree of polymorphism in an individual, which is heritable form parents to children
4. Does not code for proteins and is same in all members of the population.
Gene regulation governing lactose operon of E. coli that involves the lac 1 gene product is
1. Feedback inhibition because excess of galactosidase can switch off transcription
2. Positive an inducible because it can be induced by lactose
3. Negative and inducible because repressor protein prevents transcription
4. Negative and repressible because repressor protein prevents transcription
Which enzyme/s will be produced in a cell in which there is a nonsence mutation in the lac Y gene?
1. Lactose permease
3. Lactose permease and transacetylase
Which one of the following is not a part of a transcription ubit in DNA?
1. A promoter
2. The structural gene
3. The inducer
4. A terminator
Read the following four statement (A-D)
A. In transcription, adenosine pair with uracil
B. Regulation of lac operon by repressor is reffered to as positive regulation
C. The human genome has approximately 50,000 genes
D. Haemophilia is a sex-linked recessive disease
How many of the above statements are right?
PCR and Restriction Fragment Length Polymorphism are the methods for
1. DNA sequencing
2. Genetic fingerprinting
3. Study of enzymes
4. Genetic transformation
Which one of the following does not follow the central dogma of molecular biology?
One gene-one enzyme relationship was established for the first time in
1. Diploccus pneumoniae
2. Neurospora crassa
3. Salmonella typhimurium
4. Escherichia coli
Telomere repetitive DNA sequence control the function of eukaryote chromosomes because they:
1. Prevent chromosome loss
2. Act as replicons
3. Are RNA transcription initiator
4. Help chromosome pairing
During transcription, RNA polymerase holoenzyme binds to a gene promoter and assumes a saddle - like structure. What id it's DNA - binding sequence?
The length of DNA molecule greatly exceeds the dimensions of the nucleus in eukaryotic cells. How is this DNA accommodated?
1. Through elimination of repetitive DNA
2. Deletion of non-essential genes
3. Super-coiling in nucleosomes
4. DNA se digestion
In an inducible operon, the genes are
1. Always expressed
2. Usually not expressed unless a signal turns them 'on'
3. Usually expresses unless a signal turns them 'off'
4. Never expressed
A single strand of nucleic acid tagged with a radioactive molecule is called
4. Selectable marker
The reaction, Amino acid + ATP Aminoacyl - AMP + P-P depicts
1. Amino acid assimilation
2. Amino acid transformation
3. Amino acis activation
4. Amino acid translocation
There are special proteins that help to open up DNA double helix in front of the replication fork. These proteins are
1. DNA ligase
2. DNA topoisomerase I
3. DNA gyrase
4. DNA polymerase I
In split genes, the coding sequences are called
DNA elements, which can switch their position, are called
Irregularity is found in Drosophila during the organ differentiation. For example, in place of wing long legs are formed. Which gene is responsible?
1. Double dominant gene
2. Homeotic gene
3. Complimentary gene
4. Plastid gene
Which of the following reunites the exon segments after RNA splicing?
1. RNA polymerase
2. RNA primase
3. RNA ligase
4. RNA proteoses
During transcription, the DNA site at which RNA polymerase binds is called
Which antibiotics inhibits interaction between tRNA and mRNA during bacterial protein synthesis?
E. coli about to replicate was placed in a medium containing radioactive thymidine for five minutes. Then it was made to replicate in a normal medium. Which of the following observation shall be correct?
1. Both the strands of DNA will be radioactive
2. One strand radioactive
3. Each strand hald radioactive
4. None is radioactive