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When the phage DNA enters a bacterium, it protects itself from the viral DNA with the help of
(1) methylases
(2) endonucleases
(3) exonucleases
(4) ligases

The enzyme used in the polymerase chain reaction is a
(1) DNA dependent RNA polymerase
(2) RNA dependent DNA polymerase
(3) DNA dependent DNA polymerase
(4) RNA dependent RNA polymerase

If the required gene has been isolated by shot gun approach, care must be taken to :
(1) cut the plasmid with a different restriction enzyme than that used to obtain donor            gene
(2) cut the plasmid with the same restriction enzyme that was used to obtain donor gene
(3) avoid cutting the plasmid
(4) cut the plasmid with two different restriction enzymes

Taq polymerase is used in the polymerase chain reaction because:
(1) It replicates DNA faster than other enzymes
(2) It is the only enzyme that can replicate DNA invitro
(3) It does not require an RNA primer to function
(4) It is thermostable

A cloning vector has two antibiotic resistance genes- for tetracycline and ampicillin. A foreign DNA was inserted into the tetracycline gene Non-recombinants would survive on the medium containing
(1) ampicillin but not tetracycline
(2) tetracycline but not ampicillin
(3) both tetracycline and ampicillin
(4) neither tetracycline nor ampicillin

Using reverse transcriptase or gene synthesis has an advantage over the shot gun approach in obtaining a copy of the gene required as:
(1) the gene produced can easily be ligated to the vector
(2) the gene produced is not a split gene
(3) the gene produced does not require a promoter to be expressed
(4) the gene produced does not require an ori to replicate

Elution is
(1) separating the restricted DNA fragments on agarose gel
(2) staining the separated DNA fragments with ethidium bromide
(3) cutting out of the separated bands of DNA from the agarose gel and extracting them from the gel piece
(4) constructing rDNA by joining the purified DNA fragments to the cloning vector

When isolating the pure DNA from a bacterial  cell, the cell should not be treated with
(1) Hysozyme
(2) proteases
(3) ribonuclease
(4) deoxyribonuclease

A host cell normally does not take up a foreign DNA until it has been made competent to do so. This is because:
(1) DNA is a hydrophilic molecule
(2) DNA is a very large molecule
(3) there are no receptors for DNA on the cell membrane
(4) DNA is an inert molecule

Which of the following has highest boiling point? 

(1) 0.01 M NaCl                  
(2) 0.05 M Urea 
(3) 0.01 M MgCl₂                     
(4) 0.02 M NaCl

When 15 gram of non-volatile & non electrolyte solute is dissolved in 200 g of H₂O, then its vapour pressure is found to be 780 mm of Hg. If vapour pressure of pure solvent is 800 mm of Hg, then calculate the molecular weight of solute.
(1) 54
(2) 108
(3) 27
(4) 42

K₄Fe(CN)₆ is 60% dissociated. Value of Van’t Hoff Factor (i) is 
(1)  1.8
(2) 4.2
(3) 5.3
(4) 3.4 

Benzene & Toluene form nearly an ideal solution. The vapour pressure of Benzene & Toluene in pure state are 700 and 600 mm of Hg respectively. Total vapour pressure of solution if one mole of Benzene and 4 moles of Toluene are mixed will be
(1) 460 mm Hg
(2) 120 mm Hg
(3) 620 mm Hg
(4) 480 mm Hg

Osmotic pressure of 2% Urea Solution at 270C will be:
(1) 8.21 atm
(2) 7.52 atm
(3) 8.97 atm
(4) 7.14 atm

If λ_v, λ_x and λ_m represent the wavelength of visible light, x rays and microwaves respectively, then
(1) λ_m > λ_x > λ_v
(2) λ_v > λ_m > λ_x
(3) λ_m > λ_v > λ_x
(4) λ_v > λ_x > λ_m

The electric and the magnetic field, associated with an electromagnetic wave propagating along y-axis can be represented by
(1) E⃗ = E₀ k ̂ ,  B⃗ = B₀ i ̂
(2) E⃗ = E₀ j ̂ , B⃗ = B₀ j ̂
(3) E⃗ = E₀ j ̂ , B⃗ = B₀ k ̂
(4) E⃗ = E₀ i ̂ , B⃗ = B₀ j ̂

An electromagnetic wave going through vacuum is described by E = E₀ sin(kx – wt), B = B₀ sin (kx – wt). which of the following equation is true
(1) E₀k = B₀w
(2) E₀w = B₀k
(3) E₀B₀ = wk
(4) E₀B₀ = w/k

In a free space electron is placed in path of a plane electromagnetic wave, it will start moving along
(1) centre of earth
(2) equator of earth
(3) magnetic field
(4) Electric field

Which of the following cause "Green House Effect"
(1) Infrared rays
(2) Ultraviolet light
(3) X rays
(4) Radio waves