NEET and AIPMT NEET Zoology Biotechnology and its Application MCQ Questions Solved


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The enzyme used in the polymerase chain reaction is a :

(1) DNA dependent RNA polymerase

(2) RNA dependent DNA polymerase

(3) DNA dependent DNA polymerase

(4) RNA dependent RNA polymerase

(3) Amplifying DNA would require making DNA on the template of DNA.

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Taq polymerase is used in the polymerase chain reaction because:

(1) It replicates DNA faster than other enzymes

(2) It is the only enzyme that can replicate DNA invitro

(3) It does not require an RNA primer to function

(4) It is thermostable 

(4) PCR uses a thermal cycle for denaturation of DNA and therefore a thermostable DNA polymerase is needed.

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Plants are more easily manipulated by genetic engineering than are animals because:

(1) Plant genes do not contain introns

(2) More vectors are available to transfer rDNA into plant cells

(3) Totipotency is a feature generally associated with plant cells and not with animal cells

(4) Genes can be inserted into animal cells by microinjection.

(3) (E) Totipotency is a feature generally associated with plant cells and not with animal cells. 

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Identify the incorrectly matched pair :

(1)

 Humulin

First therapeutic rDNA product approved for human use

(2)

RNAi

Silencing of mRNA with the help of dsRNA

(3)

Rosie

Transgenic sheep producing alpha 1 antitrypsin

(4)

Golden rice

Vitamin A enriched rice variety

(3) page 213, XII NCERT

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RNAi is a mechanism to silence genes with the help of 

(1) ssRNA

(2) dsRNA

(3) ssDNA

(4) dsDNA

(2) page 209, XII NCERT

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To protect tobacco plants against Meloidegyne incognita using RNAi, nematode specific genes were introduced in the plant using:

(1) Transposons

(2) Retrotransposons

(3) Virus with RNA genome 

(4) Agrobacterium

(4) Page 209, XII NCERT

 

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A permanent cure for ADA deficiency could be:

(1) Enzyme replacement therapy

(2) Bone marrow transplant

(3) Introduction of a functional ADA cDNA into lymphocytes

(4) Introduction of gene isolate from marrow cells producing ADA into early embryonic cells

 

(4) Page 211, XII NCERT

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Taq polymerase is used in the polymerase chain reaction because:

(1) It replicates DNA faster than other enzymes

(2) It is the only enzyme that can replicate DNA invitro

(3) It does not require RNA primer to function

(4) It is thermostable   

 

 

(4) page 203, XII NCERT

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Polymerase Chain Reaction is not used in :

(1) Confirming presence of a pathogen during early infection

(2) Identifying the mutated genes in suspected cancer patients

(3) Isolating the gene of interest from host DNA to be cloned by recombinant procedures

(4) Detection of the presence of HIV in suspected AIDS patient

(3) page 212, XII NCERT

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