- 1(current)
Q. No.Consider the given two statements:
| Assertion (A): | It is desirable to use restriction enzymes that make asymmetrical cuts in construction of rDNA molecules. |
| Reason (R): | Formation of asymmetrical cuts facilitates the function of DNA ligase. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | Both (A) and (R) are False. |
| 4. | (A) is True, (R) is False. |
Subtopic: Â Restriction Enzymes - Main Enzymes |
 57%
Level 3: 35%-60%
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Identify the incorrect statement regarding restriction endonucleases used in rDNA technology?
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
| I: | They cleave dsDNA into fragments at specific recognition sites within molecules known as restriction sites. |
| II: | These enzymes are found in bacteria and provide a defense mechanism against invading viruses. |
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Â Restriction Enzymes - Main Enzymes |
 50%
Level 3: 35%-60%
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Consider the given two statements:
| Assertion (A): | Restriction enzymes are found in bacteria and provide a defense mechanism against invading viruses. |
| Reason (R): | They interact with membranes of the host bacterial cells and remove all the receptor sites by the viruses. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Â Restriction Enzymes - Main Enzymes |
 56%
Level 3: 35%-60%
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'Restriction' in Restriction enzyme refers to:
| 1. | Cleaving of phosphodiester bond in DNA by the enzyme |
| 2. | Cutting of DNA at specific position only |
| 3. | Prevention of the multiplication of bacteriophage by the host bacteria |
| 4. | All of the above |
Subtopic: Â Restriction Enzymes - Main Enzymes |
Level 3: 35%-60%
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Consider the two given statements:
| Assertion (A): | Foreign and vector DNA must be cleaved with the same restriction enzyme when making recombinant DNA. |
| Reason (R): | Each restriction enzyme cuts DNA at a specific cleavage site. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is True |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Â Restriction Enzymes - Main Enzymes |
Level 3: 35%-60%
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- 1(current)
Q. No.
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