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Q. No.'Restriction' in Restriction enzyme refers to:
1.
Cleaving of phosphodiester bond in DNA by the enzyme
2.
Cutting of DNA at specific position only
3.
Prevention of the multiplication of bacteriophage by the host bacteria
4.
All of the above
Subtopic: Â Restriction Enzymes - Main Enzymes |
Level 3: 35%-60%
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Consider the given two statements:
| Assertion (A): | Plasmid DNA is used to monitor bacterial transformation with foreign DNA. |
| Reason (R): | Plasmid is extrachromosomal DNA in some bacteria. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A) |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A) |
| 3. | (A) is True but (R) is False |
| 4. | (A) is False but (R) is True |
Subtopic: Â Cloning Vector |
Level 3: 35%-60%
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The process by which a virus transfers genetic material from one bacterium to another is called:
| 1. | Transduction | 2. | Conjugation |
| 3. | Transformation | 4. | Translation |
Subtopic: Â Tools |
 51%
Level 3: 35%-60%
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Consider the given two statements:
| Assertion (A): | It is desirable to use restriction enzymes that make asymmetrical cuts in construction of rDNA molecules. |
| Reason (R): | Formation of asymmetrical cuts facilitates the function of DNA ligase. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | Both (A) and (R) are False. |
| 4. | (A) is True, (R) is False. |
Subtopic: Â Restriction Enzymes - Main Enzymes |
 58%
Level 3: 35%-60%
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Which of the following is required in a cloning vector?
| 1. | Origin of replication, a selectable marker, and a resistance gene |
| 2. | Origin of replication, a selectable marker, and a foreign DNA insert |
| 3. | A gene for replication, a selectable marker, and an antibiotic resistance gene |
| 4. | Origin of replication, a high copy number, and an antibiotic resistance gene |
Subtopic: Â Cloning Vector |
Level 4: Below 35%
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Identify the incorrect statement regarding restriction endonucleases used in rDNA technology?
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
| I: | They cleave dsDNA into fragments at specific recognition sites within molecules known as restriction sites. |
| II: | These enzymes are found in bacteria and provide a defense mechanism against invading viruses. |
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Â Restriction Enzymes - Main Enzymes |
 51%
Level 3: 35%-60%
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Consider the given two statements:
| Assertion(A): | Any piece of DNA when linked to Ori sequence of a suitable vector can be made to replicate within the host cells. |
| Reason (R): | Ori sequence is responsible for controlling the copy number of the linked DNA. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Â Cloning Vector |
Level 3: 35%-60%
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Consider the given two statements:
| Assertion (A): | Restriction enzymes are found in bacteria and provide a defense mechanism against invading viruses. |
| Reason (R): | They interact with membranes of the host bacterial cells and remove all the receptor sites by the viruses. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Â Restriction Enzymes - Main Enzymes |
 56%
Level 3: 35%-60%
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What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?
Choose the correct answer from the options given below:
| A: | The piece of DNA would be able to multiply itself independently in the progeny cells of the organism. |
| B: | It may get integrated into the genome of the recipient. |
| C: | It may multiply and be inherited along with the host DNA. |
| D: | The alien piece of DNA is not an integral part of chromosome. |
| E: | It shows ability to replicate. |
| 1. | D and E only | 2. | B and C only |
| 3. | A and E only | 4. | A and B only |
Subtopic: Â Principles of Biotechnology |
 60%
Level 2: 60%+
NEET - 2024
Hints
Consider the two given statements:
| Assertion (A): | Foreign and vector DNA must be cleaved with the same restriction enzyme when making recombinant DNA. |
| Reason (R): | Each restriction enzyme cuts DNA at a specific cleavage site. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is True |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Â Restriction Enzymes - Main Enzymes |
Level 3: 35%-60%
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