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Q. No.
Assertion (A):
Synthetic oligonucleotide polymers are used during annealing in a PCR.
Reason (R):
The primers bind to the double stranded DNA at their complementary regions.
1.
Both (A) and (R) are True and (R) is the correct explanation of (A).
2.
Both (A) and (R) are True and (R) is not the correct explanation of (A).
3.
(A) is True but (R) is False.
4.
(A) is False but (R) is True.
Subtopic: Â Polymerase Chain Reaction: PCR |
Level 3: 35%-60%
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Which of the following can replicate within bacterial cells independent of the control of chromosomal DNA?
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
| I: | Plasmid |
| II: | Phage DNA |
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Â Cloning Vector |
 55%
Level 3: 35%-60%
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A plasmid has two genes providing resistance to antibiotics A and B respectively. A foreign DNA has been inserted within the gene for resistance to antibiotic A:
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
| I. | Non-Transformants will not survive in a medium containing antibiotic A and in a medium containing antibiotic B. |
| II. | Recombinant transformants will survive in a medium containing antibiotic B but not in a medium containing antibiotic A. |
| III. | Non-recombinant transformants will survive in a medium containing antibiotic A and in a medium containing antibiotic B. |
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
Subtopic: Â Tools: Vectors: I |
Level 3: 35%-60%
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Consider the two given statements:
| Assertion (A): | Foreign and vector DNA must be cleaved with the same restriction enzyme when making recombinant DNA. |
| Reason (R): | Each restriction enzyme cuts DNA at a specific cleavage site. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is True |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Â Restriction Enzymes - Main Enzymes |
Level 3: 35%-60%
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Select the correct match w.r.t steps involved in PCR.
| 1. | Denaturation | - | Phosphodiester bonds break |
| 2. | Annealing | - | Primers anneal at 5' end of DNA template |
| 3. | Extension | - | Taq polymerase forms hydrogen bonds between adjacent nucleotides of growing DNA strand |
| 4. | Extension | - | Thermostable DNA polymerase adds nucleotide to 3' end of primers |
Subtopic: Â Polymerase Chain Reaction: PCR |
Level 3: 35%-60%
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Consider the given two statements:
| Assertion (A): | One antibiotic resistance gene of vector pBR322 helps in selecting the transformants and the other one in selecting recombinants. |
| Reason (R): | The non-recombinants can grow on both antibiotic-rich media while the recombinants grow only on single medium due to insertional inactivation. |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of the (A). |
| 2. | Both (A) and (R) are True and the (R) is not the correct explanation of the (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are False. |
Subtopic: Â Cloning Vector |
Level 3: 35%-60%
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Consider the given two statements:
| Assertion: (A) | We cannot see pure DNA fragments in the visible light under microscope. |
| Reason: (R) | The DNA fragments separate according to their shape only and are too small to be seen in the gel electrophoresis. |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of the (A). |
| 2. | Both (A) and (R) are True and the (R) is not the correct explanation of the (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are False. |
Subtopic: Â Separation and Isolation of DNA fragments |
 51%
Level 3: 35%-60%
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Consider the given two statements:
| Assertion (A): | Restriction enzymes are found in bacteria and provide a defense mechanism against invading viruses. |
| Reason (R): | They interact with membranes of the host bacterial cells and remove all the receptor sites by the viruses. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Â Restriction Enzymes - Main Enzymes |
 55%
Level 3: 35%-60%
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Consider the given two statements:
| Assertion(A): | Any piece of DNA when linked to Ori sequence of a suitable vector can be made to replicate within the host cells. |
| Reason (R): | Ori sequence is responsible for controlling the copy number of the linked DNA. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Â Cloning Vector |
Level 3: 35%-60%
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Match List-I with List-II
Choose the correct answer from the options given below:
| List-I | List-II | ||
| (A) | Kanamycin | (I) | Delivers genes into animal cells |
| (B) | ClaI | (II) | Selectable marker |
| (C) | Disarmed retroviruses | (III) | Restriction site |
| (D) | Kanamycin Rgene | (IV) | Antibiotic resistance |
Choose the correct answer from the options given below:
| Options: | (A) | (B) | (C) | (D) |
| 1. | (II) | (III) | (I) | (IV) |
| 2. | (III) | (I) | (IV) | (II) |
| 3. | (IV) | (III) | (I) | (II) |
| 4. | (II) | (IV) | (I) | (III) |
Subtopic: Â Cloning Vector |
 55%
Level 3: 35%-60%
NEET - 2023
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