Q. No.DNA fragments are:
1. Negatively charged
2. Neutral
3. Either positively charged depending on their size
4. Positively charged
A gene whose expression helps to identify transformed cell is known as:
1. Vector
2. Plasmid
3. Structural gene
4. Selectable marker
What is the criterion for the movement of DNA fragments on agarose gel during gel electrophoresis?
| 1. | The smaller the fragment size, the farther it moves |
| 2. | Positively charged fragments move to farther end |
| 3. | Negatively charged fragments do not move |
| 4. | The larger the fragment size, the farther it moves |
2. DNase I
3. RNase
4. Hind II
1. Circular structure
2. Transferable
3. Single-stranded
4. Independent replication
2. Bacillius subtilis
3. Pseudomonas putida
4. Thermus aquaticus
The cutting of DNA at specific locations became possible with the discovery of?
1. Restriction enzymes
2. Probes
3. Selectable markets
4. ligases
Which vector can clone only a small fragment of DNA?
1. Bacterial artificial chromosome
2. Yeast artificial chromosome
3. Plasmid
4. Cosmid
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:
1. Polymerase chain reaction
2. Electrophoresis
3. Restriction mapping
4. Centrifugation
1. Ori-original restriction enzyme
2. Rop-reduced osmotic pressure
3. Hind III, Eco RI-selectable markers
4. AmpR, tetiR-antibiotic resistance genes
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