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Q. No.The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate.
Given below are two statements about this method:
Statement I:
The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies.
Statement II:
The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies.
1.
Statement I is correct; Statement II is incorrect
2.
Statement I is incorrect; Statement II is correct
3.
Both Statement I and Statement II are correct
4.
Both Statement I and Statement II are incorrect
Subtopic: Selection of Recombinant Transformants |
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Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below:
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below:
| 1. | D and E only | 2. | B and C only |
| 3. | C and D only | 4. | A and B only |
Subtopic: Tools |
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Given below are two statements :
In the light of the above statements, choose the most appropriate answer from the options given below :
| Statement I : | The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. |
| Statement II : | Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel. |
| 1. | Statement I is correct but statement II is incorrect |
| 2. | Statement I is incorrect but statement II is correct |
| 3. | Both statement I and statement II are correct |
| 4. | Both statement I and statement II are incorrect |
Subtopic: Restriction Enzymes - Main Enzymes |
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Identify the part of a bio-reactor which is used as a foam braker from the given figure.
| 1. | D | 2. | C |
| 3. | A | 4. | B |
Subtopic: Bioreactors/Downstream Processing |
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Polymerase chain reaction (PCR) amplifies DNA following the equation.
| 1. | \(2n+1\) | 2. | \(2N^2\) |
| 3. | \(N^2\) | 4. | \(2^n\) |
Subtopic: Polymerase Chain Reaction: PCR |
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In the above-represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
| 1. | White color colonies will be selected. |
| 2. | Blue color colonies grown on ampicillin plates can be selected. |
| 3. | Using an ampicillin- & tetracycline-containing medium plate. |
| 4. | Blue color colonies will be selected. |
Subtopic: Cloning Vector |
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Following are the steps involved in the process of PCR.
A. Annealing
B. Amplification (~1 billion times)
C. Denaturation
D. Treatment with Taq polymerase and deoxynucleotides
E. Extension
Choose the correct sequence of steps of PCR from the options given below :
1. C → A → D → E → B
2. A → B → E → D → C
3. A → C → E → D → B
4. D → B → E → C → A
A. Annealing
B. Amplification (~1 billion times)
C. Denaturation
D. Treatment with Taq polymerase and deoxynucleotides
E. Extension
Choose the correct sequence of steps of PCR from the options given below :
1. C → A → D → E → B
2. A → B → E → D → C
3. A → C → E → D → B
4. D → B → E → C → A
Subtopic: Polymerase Chain Reaction: PCR |
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Which of the following are correct about EcoRI?
Choose the correct answer from the options given below:
| A. | Cut the DNA with blunt end |
| B. | Cut the DNA with sticky end |
| C. | Recognises a specific palindromic sequence. |
| D. | Cut the DNA between the base G and A where it encounters the DNA sequence 'GAATTC' |
| E. | Exonuclease |
| 1. | B,C,E only | 2. | A,D,E only |
| 3. | A,C,D only | 4. | B,C,D only |
Subtopic: Restriction Enzymes - Main Enzymes |
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Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with:
| 1. | Hind II, Hind II | 2. | Hind II, Alu I |
| 3. | Hind II, EcoR I | 4. | Hind II, Bam HI |
Subtopic: Tools: Enzymes: I | Tools: Enzymes: II |
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Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tetR) gene in the pBR322 cloning vector.
1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI
1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI
Subtopic: Cloning Vector |
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